From the identification of gene organization of alpha conotoxins to the cloning of novel toxins

	From the identification of gene organization of alpha conotoxins to the cloning of novel toxins
	Yuan, DD; Han, YH; Wang, CG; Chi, CW
刊名	TOXICON
	2007
卷号	49 期号:8 页码:1135-1149
关键词	alpha conotoxin cDNA cloning gene cloning intron evolution
通讯作者	Wang, CG (reprint author), Tongji Univ, Inst Protein Res, 1239 Siping Rd, Shanghai 200092, Peoples R China.,chunguangwang@mail.tongji.edu.en ; chi@sunm.shcnc.ac.cn
英文摘要	In the venoms of cone snails, alpha conotoxins are competitive antagonists of nicotinic acetylcholine receptors. Eleven novel cDNA and eight partial gene sequences (including two pseudogenes) of alpha conotoxins were identified from five species of cone snail. As expected, every cDNA encodes a precursor of prepropeptide. In all the partial genes of a conotoxins identified, there is a long intron inserted at a fixed position in the pro-region, dividing the encoding region into two exons. The mutation rate in exon I (encoding the signal peptide and a part of pro-region) is much lower than that in exon II (encoding the other part of pro-region, the mature peptide and 3' untranslational region). Interestingly, the sequences at the 5' and 3' end of introns are highly conserved. In addition, in the identified introns exist long dinucleotide (e.g. "GT", "CA") or trinucleotide ("CAT") repeats. In the special case of Pu1.1, there are five almost identical repeats of a 150bp sequence in the long intron. Taking advantage of the conserved 3' end sequence of intron, 16 alpha. conotoxins, as well as a pseudogene and three kappa A conotoxins, were identified from their genomic DNAs. Based on the comparison of these cDNA and gene sequences, a hypothesis of the alpha conotoxin evolution was proposed. (c) 2007 Elsevier Ltd. All rights reserved.
学科主题	Pharmacology & Pharmacy; Toxicology
类目[WOS]	Pharmacology & Pharmacy ; Toxicology
关键词[WOS]	NICOTINIC ACETYLCHOLINE-RECEPTORS ; SUPERFAMILY CONOTOXINS ; CONUS-GEOGRAPHUS ; VENOM PEPTIDES ; A-SUPERFAMILY ; CDNA CLONING ; CHANNEL ; TARGETS ; DIVERGENCE ; SEQUENCES
收录类别	SCI
语种	英语
WOS记录号	WOS:000247711400008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1592]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yuan, DD,Han, YH,Wang, CG,et al. From the identification of gene organization of alpha conotoxins to the cloning of novel toxins[J]. TOXICON,2007,49(8):1135-1149.
APA	Yuan, DD,Han, YH,Wang, CG,&Chi, CW.(2007).From the identification of gene organization of alpha conotoxins to the cloning of novel toxins.TOXICON,49(8),1135-1149.
MLA	Yuan, DD,et al."From the identification of gene organization of alpha conotoxins to the cloning of novel toxins".TOXICON 49.8(2007):1135-1149.