Disruption of imprinting and aberrant embryo development in completely inbred embryonic stem cell-derived mice

	Disruption of imprinting and aberrant embryo development in completely inbred embryonic stem cell-derived mice
	Deng, T; Kuang, Y; Zhang, D; Wang, L; Sun, R; Xu, GJ; Wang, ZG; Fei, J
刊名	DEVELOPMENT GROWTH & DIFFERENTIATION
	2007
卷号	49 期号:7 页码:603-610
关键词	DNA methylation embryo development epigenetic genome imprinting inbred ES cell
通讯作者	Kuang, Y (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai Inst Biol Sci, Shanghai, Peoples R China.,kuangying01@yahoo.com.cn ; jfei@sibs.ac.cn
英文摘要	The completely embryonic stem (ES) cell-derived mice (ES mice) produced by tetraploid embryo complementation provide us with a rapid and powerful approach for functional genome analysis. However, inbred ES cell lines often fail to generate ES mice. The genome of mouse ES cells is extremely unstable during in vitro culture and passage, and the expression of the imprinted genes is most likely to be affected. Whether the ES mice retain or repair the abnormalities of the donor ES cells has still to be determined. Here we report that the inbred ES mice were efficiently produced with the inbred ES cell line (SCR012). The ES fetuses grew more slowly before day 17.5 after mating, but had an excessive growth from day 17.5 to birth. Five imprinted genes examined (H19, Igf2, Igf2r, Peg1, Peg3) were expressed abnormally in ES fetuses. Most remarkably, the expression of H19 was dramatically repressed in the ES fetuses through the embryo developmental stage, and this repression was associated with abnormal biallelic methylation of the H19 upstream region. The altered methylation pattern of H19 was further demonstrated to have arisen in the donor ES cells and persisted on in vivo differentiation to the fetal stage. These results indicate that the ES fetuses did retain the epigenetic alterations in imprinted genes from the donor ES cells.
学科主题	Cell Biology; Developmental Biology
类目[WOS]	Cell Biology ; Developmental Biology
关键词[WOS]	TETRAPLOID EMBRYOS ; BECKWITH-WIEDEMANN ; TARGETED MUTATION ; FETAL DEVELOPMENT ; MOUSE FETUSES ; ES CELLS ; GENERATION ; COMPLEMENTATION ; EXPRESSION ; LETHALITY
收录类别	SCI
语种	英语
WOS记录号	WOS:000248967100006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1581]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Deng, T,Kuang, Y,Zhang, D,et al. Disruption of imprinting and aberrant embryo development in completely inbred embryonic stem cell-derived mice[J]. DEVELOPMENT GROWTH & DIFFERENTIATION,2007,49(7):603-610.
APA	Deng, T.,Kuang, Y.,Zhang, D.,Wang, L.,Sun, R.,...&Fei, J.(2007).Disruption of imprinting and aberrant embryo development in completely inbred embryonic stem cell-derived mice.DEVELOPMENT GROWTH & DIFFERENTIATION,49(7),603-610.
MLA	Deng, T,et al."Disruption of imprinting and aberrant embryo development in completely inbred embryonic stem cell-derived mice".DEVELOPMENT GROWTH & DIFFERENTIATION 49.7(2007):603-610.