Downregulation of NFAT5 by RNA interference reduces monoclonal antibody productivity of hybridoma cells

	Downregulation of NFAT5 by RNA interference reduces monoclonal antibody productivity of hybridoma cells
	Ju, JH; Zou, K; Xie, H
刊名	CELL RESEARCH
	2007
卷号	17 期号:3 页码:264-270
关键词	antibody formation hybridomas NFAT transcription factors RNA interference
通讯作者	Xie, H (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,hxie@sibs.ac.cn
英文摘要	Hybridoma cells display an increase in antibody productivity following exposure to hypertonic conditions. However, the underlying mechanism is not well understood. In the present study, we hypothesize that the nuclear factor of activated T cells 5 (NFAT5)/tonicity enhancer binding protein (TonEBP) functions to increase the antibody productivity of hybridoma cells. NFAT5 is an osmosensitive mammalian transcription factor. However, its ubiquitous expression in various organs that are not bathed in hypertonic milieu suggests that NFAT5 may also regulate cell growth and function under isotonic conditions. In this study, we examined the expression of NFAT5 in hybridoma cells by Western blot analysis, and found that it increased significantly in hypertonic medium. To further define the function of NFAT5 in hybridoma cells, RNA interference technique was used to downregulate the expression of NFAT5 in SGB-8 cells (a hybridoma cell line). In isotonic medium, antibody productivity of hybridoma cells was reduced by downregulation of NFAT5 while cell proliferation was not influenced. The results presented here demonstrate that NFAT5 not only plays an important role in osmotic stress response pathway in hybridoma cells but also is essential for optimal antibody productivity.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	ENHANCER-BINDING PROTEIN ; OSMOTIC-STRESS ; TRANSCRIPTION FACTOR ; EXPRESSION ; HYPERTONICITY ; TONICITY ; GENES ; ACTIVATION ; RESPONSES
收录类别	SCI
语种	英语
WOS记录号	WOS:000246128100008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1541]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Ju, JH,Zou, K,Xie, H. Downregulation of NFAT5 by RNA interference reduces monoclonal antibody productivity of hybridoma cells[J]. CELL RESEARCH,2007,17(3):264-270.
APA	Ju, JH,Zou, K,&Xie, H.(2007).Downregulation of NFAT5 by RNA interference reduces monoclonal antibody productivity of hybridoma cells.CELL RESEARCH,17(3),264-270.
MLA	Ju, JH,et al."Downregulation of NFAT5 by RNA interference reduces monoclonal antibody productivity of hybridoma cells".CELL RESEARCH 17.3(2007):264-270.