Potent antitumor efficacy of XAF1 delivered by conditionally replicative adenovirus vector via caspase-independent apoptosis

	Potent antitumor efficacy of XAF1 delivered by conditionally replicative adenovirus vector via caspase-independent apoptosis
	Qi, R; Gu, J; Zhang, Z; Yang, K; Li, B; Fan, J; Wang, C; He, Z; Qiao, L; Lin, Z
刊名	CANCER GENE THERAPY
	2007
卷号	14 期号:1 页码:82-90
关键词	XAF1 ZD55 caspase-independent apoptosis gene-virotherapy
通讯作者	Liu, XY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,xyliu@sibs.ac.cn
英文摘要	XAF1 is a newly identified tumor-suppressor gene that can antagonize XIAP and sensitize cells to other cell death triggers. In this study, we utilized ZD55, a conditionally replicative adenovirus ( CRAd) similar to ONYX-015 as the vector to transfer XAF1 into the tumor cells to evaluate its antitumor efficacy in vitro and in vivo. Potent and specific cytopathic effect ( CPE) was observed upon infection with ZD55-XAF1 in tumor cell lines. Importantly, ZD55-XAF1 exhibited a superior suppression of tumor growth in an animal model of colorectal carcinoma in nude mice compared with Ad-XAF1 ( E1-deleted replication-defective viral) and ONYX-015. Complete eradication of the established tumors was observed in four of eight mice. Our data also showed that infection with ZD55-XAF1 resulted in caspase-independent apoptosis. Although caspase-3, poly( ADP-ribose) polymerase were mildly activated in response to ZD55-XAF1 infection, pretreatment with pan-caspase inhibitor hardly influence its apoptosis-inducing activity. In summary, our study strongly suggested that ZD55-XAF1 could serve as an effective gene-virotherapy strategy and has highly potential against human cancers.
学科主题	Biotechnology & Applied Microbiology; Oncology; Genetics & Heredity; Research & Experimental Medicine
类目[WOS]	Biotechnology & Applied Microbiology ; Oncology ; Genetics & Heredity ; Medicine, Research & Experimental
关键词[WOS]	XIAP-ASSOCIATED FACTOR-1 ; X-LINKED INHIBITOR ; GENE-THERAPY ; CELL-DEATH ; TRANSGENE EXPRESSION ; CANCER-THERAPY ; IDENTIFICATION ; MECHANISMS ; TARGET ; TRAIL
收录类别	SCI
语种	英语
WOS记录号	WOS:000242850400010
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1513]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Qi, R,Gu, J,Zhang, Z,et al. Potent antitumor efficacy of XAF1 delivered by conditionally replicative adenovirus vector via caspase-independent apoptosis[J]. CANCER GENE THERAPY,2007,14(1):82-90.
APA	Qi, R.,Gu, J.,Zhang, Z.,Yang, K.,Li, B.,...&Liu, XY.(2007).Potent antitumor efficacy of XAF1 delivered by conditionally replicative adenovirus vector via caspase-independent apoptosis.CANCER GENE THERAPY,14(1),82-90.
MLA	Qi, R,et al."Potent antitumor efficacy of XAF1 delivered by conditionally replicative adenovirus vector via caspase-independent apoptosis".CANCER GENE THERAPY 14.1(2007):82-90.