alpha 4/7-conotoxin Lp1.1 is a novel antagonist of neuronal nicotinic acetylcholine receptors

	alpha 4/7-conotoxin Lp1.1 is a novel antagonist of neuronal nicotinic acetylcholine receptors
	Peng, C; Han, YH; Sanders, T; Chew, G; Liu, J; Hawrot, E; Chi, CW; Wang, CG
刊名	PEPTIDES
	2008
卷号	29 期号:10 页码:1700-1707
关键词	Conus alpha-Conotoxins Neuronal nicotinic acetylcholine receptor subtypes Xenopus oocytes
通讯作者	Wang, CG (reprint author), Tongji Univ, Inst Prot Res, Sch Life Sci & Technol, 1239 Siping Rd, Shanghai 200092, Peoples R China.,chunguangwang@mail.tongji.edu.cn
英文摘要	Cone snails comprise approximately 700 species of venomous molluscs which have evolved the ability to generate multiple toxins with varied and exquisite selectivity. a-Conotoxin is a powerful tool for defining the composition and function of nicotinic acetylcholine receptors which play a crucial role in excitatory neurotransmission and are important targets for drugs and insecticides. An alpha 4/7 conotoxin, Lp1.1, originally identified by cDNA and genomic DNA cloning from Conus leopardus, was found devoid of the highly conserved Pro residue in the first intercysteine loop. To further study this toxin, alpha-Lp1.1 was chemically synthesized and refolded into its globular disulfide isomer. The synthetic Lp1.1 induced seizure and paralysis on freshwater goldfish and selectively reversibly inhibited ACh-evoked currents in Xenopus oocytes expressing rat alpha 3 beta 2 and alpha 6 alpha 3 beta 2 nAChRs. Comparing the distinct primary structure with other functionally related alpha-conotoxins could indicate structural features in Lp1.1 that may be associated with its unique receptor recognition profile. (C) 2008 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Pharmacology & Pharmacy
类目[WOS]	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]	ALPHA-CONOTOXIN PNIA ; CRYSTAL-STRUCTURE ; CONUS-GEOGRAPHUS ; DRUG LEADS ; SUBTYPES ; PEPTIDE ; SELECTIVITY ; TOOLS ; NACHR ; VENOM
收录类别	SCI
语种	英语
WOS记录号	WOS:000260284100009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1447]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Peng, C,Han, YH,Sanders, T,et al. alpha 4/7-conotoxin Lp1.1 is a novel antagonist of neuronal nicotinic acetylcholine receptors[J]. PEPTIDES,2008,29(10):1700-1707.
APA	Peng, C.,Han, YH.,Sanders, T.,Chew, G.,Liu, J.,...&Wang, CG.(2008).alpha 4/7-conotoxin Lp1.1 is a novel antagonist of neuronal nicotinic acetylcholine receptors.PEPTIDES,29(10),1700-1707.
MLA	Peng, C,et al."alpha 4/7-conotoxin Lp1.1 is a novel antagonist of neuronal nicotinic acetylcholine receptors".PEPTIDES 29.10(2008):1700-1707.