Symbiotic plasmid is required for NolR to fully repress nodulation genes in Rhizobium leguminosarum A34

	Symbiotic plasmid is required for NolR to fully repress nodulation genes in Rhizobium leguminosarum A34
	Li, FQ; Hou, BH; Hong, GF
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2008
卷号	40 期号:10 页码:901-907
关键词	NolR symbiotic plasmid nod box
通讯作者	Hong, GF (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,gfhong@sibs.ac.cn
英文摘要	NolR is a regulator of nodulation genes present in Rhizobium and Sinorhizobium. However, the mechanism by which NolR participates in the inducible transcription of nodulation genes remains unclear. To investigate whether there are other factors regulating the function of NolR, an insertion mutant of NolR in Rhizobium leguminosarum strain 8401, which lacks the symbiotic plasmid, was constructed by homologous recombination. We investigated the effects of NolRinactivation on the expression of nodulation genes. Three inducible nodulation genes (nodA, nodF and nodM) were expressed constitutively in NolR mutant, MR114. Our results suggested that the symbiotic plasmid is required for NolR to fully repress nodulation genes in Rhizobium leguminosarum A34. In addition, MR114 has provided a useful tool for further study of molecular interactions between NolR and other factors.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	BROAD HOST RANGE ; BRADYRHIZOBIUM-JAPONICUM ; SINORHIZOBIUM-MELILOTI ; EXPRESSION ; NODD ; IDENTIFICATION ; SYSTEM ; RECOGNITION ; PROTEINS ; MUTATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000260131100009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1443]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, FQ,Hou, BH,Hong, GF. Symbiotic plasmid is required for NolR to fully repress nodulation genes in Rhizobium leguminosarum A34[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2008,40(10):901-907.
APA	Li, FQ,Hou, BH,&Hong, GF.(2008).Symbiotic plasmid is required for NolR to fully repress nodulation genes in Rhizobium leguminosarum A34.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,40(10),901-907.
MLA	Li, FQ,et al."Symbiotic plasmid is required for NolR to fully repress nodulation genes in Rhizobium leguminosarum A34".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 40.10(2008):901-907.