Expression and characterization of rice putative PAUSED gene

	Expression and characterization of rice putative PAUSED gene
	Yao, CG; Ge, LF; Li, W; Zhao, BT; Li, CQ; Ruan, KC; Lin, HX; Jin, YX
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2008
卷号	40 期号:10 页码:893-900
关键词	PAUSED tRNA export development defects rice
通讯作者	Ruan, KC (reprint author), Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,kcruan@sibs.ac.cn ; yxjin@sibs.ac.cn
英文摘要	In Arabidopsis, PAUSED (PSD) encodes the ortholog of los1p/exportin-t, which mediates the nuclear export of transfer RNA (tRNA) in yeast and mammals. However, in monocot plants such as rice, knowledge of the corresponding ortholog is limited, and its effects on growth development and productivity remain unknown. In this study, we verified a rice transfer-DNAinsertional mutant psd line and analyzed its phenotypes; the mutant displayed severe morphological defects including retarded development and low fertility compared with wild-type rice. Examining intronless tRNA-Tyr and intron-containing pre-tRNA-Ala expression levels in cytoplasmic and nuclear fraction with Northern blot analysis between wild-type and mutant leaf tissue suggested that rice PSD might be involved in tRNA export from the nucleus to the cytoplasm. Additionally, reverse transcription-polymerase chain reaction analysis revealed that PSD transcript was expressed throughout normal rice plant development, and subcellular localization assays showed that rice PSD protein was present in both the nucleus and cytoplasm. In summary, our data implied that the putative PSD gene might be indispensable for normal rice development and its function might be the same as that of Arabidopsis PSD.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	DNA INSERTIONAL MUTAGENESIS ; NUCLEAR EXPORT RECEPTOR ; TRANSFER-RNA ; TAGGING LINES ; ARABIDOPSIS ; IDENTIFICATION ; GENERATION ; DATABASE ; PORE
收录类别	SCI
语种	英语
WOS记录号	WOS:000260131100008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1442]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yao, CG,Ge, LF,Li, W,et al. Expression and characterization of rice putative PAUSED gene[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2008,40(10):893-900.
APA	Yao, CG.,Ge, LF.,Li, W.,Zhao, BT.,Li, CQ.,...&Jin, YX.(2008).Expression and characterization of rice putative PAUSED gene.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,40(10),893-900.
MLA	Yao, CG,et al."Expression and characterization of rice putative PAUSED gene".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 40.10(2008):893-900.