Significant antitumor activity of oncolytic adenovirus expressing human interferon-beta for hepatocellular carcinoma

	Significant antitumor activity of oncolytic adenovirus expressing human interferon-beta for hepatocellular carcinoma
	He, LF; Gu, JF; Tang, WH; Fan, JK; Wei, N; Zou, WG; Zhang, YH; Zhao, LL; Liu, XY
刊名	JOURNAL OF GENE MEDICINE
	2008
卷号	10 期号:9 页码:983-992
关键词	oncolytic adenovirus interferon-beta targeting gene-virotherapy hepatocellular carcinoma
通讯作者	Liu, XY (reprint author), Chinese Acad Sci, Lab Canc Therapy, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,xyliu@sibs.ac.cn
英文摘要	Background Human interferon-beta (IFN-beta) has been widely used in gene therapy for its antitumor activity but its therapeutic effect is limited. The conditionally replicative adenovirus ONYX-015 (a E1B-55-kDa-deleted adenovirus) targets well to tumor cells, but is not potent enough to cause significant tumor regression. To solve these problems, a tumor-selective replicating adenovirus expressing IFN-beta was constructed in this study. Methods The oncolytic adenoviruses were generated by homologous recombination in packaging cells. The expression of the IFN-beta protein was detected by enzyme-linked immunosorbent assay (ELISA). The antitumor efficacy of ZD55-IFN-beta was evaluated in cell lines and human hepatocellular carcinoma xenografts in nude mice. Results ZD55-IFN-beta can express much more IFN-beta than Ad-IFN-beta because of the replication of the ZD55 vector. Our data showed that ZD55-IFN-beta could exert a strong cytopathic effect on tumor cells (about 100-fold higher than Ad-IFN-beta or ONYX-015). Moreover, no obvious cytotoxic or apoptotic effects were detected in normal cells infected with ZD55-IFN-beta. Conclusions The antitumor efficacy of IFN-beta could be significantly improved due to the increased gene expression level from the tumor-selective replicating vector. The oncolytic adenovirus expressing IFN-beta may provide a novel approach for cancer gene therapy. Copyright (C) 2008 John Wiley & Sons, Ltd.
学科主题	Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine
类目[WOS]	Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental
关键词[WOS]	APOPTOSIS-INDUCING LIGAND ; TARGETING GENE-VIROTHERAPY ; REPLICATING ADENOVIRUS ; CANCER-CELLS ; NECK-CANCER ; TUMOR ; THERAPY ; ONYX-015 ; TRIAL ; REGRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000260361600004
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1429]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	He, LF,Gu, JF,Tang, WH,et al. Significant antitumor activity of oncolytic adenovirus expressing human interferon-beta for hepatocellular carcinoma[J]. JOURNAL OF GENE MEDICINE,2008,10(9):983-992.
APA	He, LF.,Gu, JF.,Tang, WH.,Fan, JK.,Wei, N.,...&Liu, XY.(2008).Significant antitumor activity of oncolytic adenovirus expressing human interferon-beta for hepatocellular carcinoma.JOURNAL OF GENE MEDICINE,10(9),983-992.
MLA	He, LF,et al."Significant antitumor activity of oncolytic adenovirus expressing human interferon-beta for hepatocellular carcinoma".JOURNAL OF GENE MEDICINE 10.9(2008):983-992.