Quality of embryonic bodies and seeding density effects on neural differentiation of mouse embryonic stem cells

	Quality of embryonic bodies and seeding density effects on neural differentiation of mouse embryonic stem cells
	Zhou, JM; Xing, FY; Shi, JJ; Fang, ZF; Chen, XJ; Chen, F
刊名	CELL BIOLOGY INTERNATIONAL
	2008
卷号	32 期号:9 页码:1169-1175
关键词	Embryonic stem cells Embryonic bodies Neural differentiation Cell density
通讯作者	Chen, XJ (reprint author), Shanghai Jiao Tong Univ, Dept Lab Anim Sci, Sch Med, 227 S Chong Qing Rd, Shanghai 200025, Peoples R China.,cdbxh@yahoo.cn
英文摘要	Mouse embryonic stem (ES) cells can be differentiated into neural lineage cells, but the differentiation efficiency remains low. This study revealed two important factors that influence the neural differentiation efficiency of mouse ES cells: the first is the quality of embryonic bodies (EBs); good quality of EBs consistently originated from a suspension culture of 1 x 10(5) ES cells/ml serum-free chemically defined neural inducing medium and they exhibited a smooth round shape, with a dark central region surrounded by a light band. Such EBs are capable of attaining high neural differentiation efficiency. However, poor quality EBs originated from a suspension culture of 1 x 10(6) ES cells/ml serum-free chemically defined neural inducing medium and exhibited an irregular shape or adhered to the bottom of the dish; they displayed low neural differentiation efficiency. The second factor is the seeding density of EBs: a low seeding density (5 EBs/cm(2)) induced cells to differentiate into a more caudalized subtypes compared to the cells obtained from high seeding density (20 EBs/cm(2)). These findings provided fresh insight into the neural induction of mouse ES cells. (C) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	DIRECTED DIFFERENTIATION ; DOPAMINERGIC-NEURONS ; ES CELLS ; MIDBRAIN ; SPECIFICATION ; PRECURSORS ; MESODERM ; CULTURE ; NURR1 ; GENE
收录类别	SCI
语种	英语
WOS记录号	WOS:000260671700017
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1426]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhou, JM,Xing, FY,Shi, JJ,et al. Quality of embryonic bodies and seeding density effects on neural differentiation of mouse embryonic stem cells[J]. CELL BIOLOGY INTERNATIONAL,2008,32(9):1169-1175.
APA	Zhou, JM,Xing, FY,Shi, JJ,Fang, ZF,Chen, XJ,&Chen, F.(2008).Quality of embryonic bodies and seeding density effects on neural differentiation of mouse embryonic stem cells.CELL BIOLOGY INTERNATIONAL,32(9),1169-1175.
MLA	Zhou, JM,et al."Quality of embryonic bodies and seeding density effects on neural differentiation of mouse embryonic stem cells".CELL BIOLOGY INTERNATIONAL 32.9(2008):1169-1175.