Recognition of tRNA(Leu) by Aquifex aeolicus leucyl-tRNA synthetase during the aminoacylation and editing steps

	Recognition of tRNA(Leu) by Aquifex aeolicus leucyl-tRNA synthetase during the aminoacylation and editing steps
	Yao, P; Zhu, B; Jaeger, S; Eriani, G; Wang, ED
刊名	NUCLEIC ACIDS RESEARCH
	2008
卷号	36 期号:8 页码:2728-2738
通讯作者	Wang, ED (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Grad Sch,State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,edwang@sibs.ac.cn
英文摘要	Recognition of tRNA by the cognate aminoacyl-tRNA synthetase during translation is crucial to ensure the correct expression of the genetic code. To understand tRNA(Leu) recognition sets and their evolution, the recognition of tRNA(Leu) by the leucyl-tRNA synthetase (LeuRS) from the primitive hyperthermophilic bacterium Aquifex aeolicus was studied by RNA probing and mutagenesis. The results show that the base A73; the core structure of tRNA formed by the tertiary interactions U8A14, G18U55 and G19C56; and the orientation of the variable arm are critical elements for tRNA(Leu) aminoacylation. Although dispensable for aminoacylation, the anticodon arm carries discrete editing determinants that are required for stabilizing the conformation of the post-transfer editing state and for promoting translocation of the tRNA acceptor arm from the synthetic to the editing site.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	ESCHERICHIA-COLI ; DISCRIMINATOR BASES ; HUMAN TRNA(SER) ; IN-VITRO ; IDENTITY ; LEUCINE ; CONFORMATION ; COMPILATION ; SEQUENCES ; SELECTION
收录类别	SCI
语种	英语
WOS记录号	WOS:000255759600027
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1419]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yao, P,Zhu, B,Jaeger, S,et al. Recognition of tRNA(Leu) by Aquifex aeolicus leucyl-tRNA synthetase during the aminoacylation and editing steps[J]. NUCLEIC ACIDS RESEARCH,2008,36(8):2728-2738.
APA	Yao, P,Zhu, B,Jaeger, S,Eriani, G,&Wang, ED.(2008).Recognition of tRNA(Leu) by Aquifex aeolicus leucyl-tRNA synthetase during the aminoacylation and editing steps.NUCLEIC ACIDS RESEARCH,36(8),2728-2738.
MLA	Yao, P,et al."Recognition of tRNA(Leu) by Aquifex aeolicus leucyl-tRNA synthetase during the aminoacylation and editing steps".NUCLEIC ACIDS RESEARCH 36.8(2008):2728-2738.