Identification of a novel S-superfamily conotoxin from vermivorous Conus caracteristicus

	Identification of a novel S-superfamily conotoxin from vermivorous Conus caracteristicus
	Liu, L; Wu, XC; Yuan, DD; Chi, CW; Wang, CG
刊名	TOXICON
	2008
卷号	51 期号:8 页码:1331-1337
关键词	S-superfamily conotoxin sequence diversity cysteine framework CDNA cloning signal peptide
通讯作者	Wang, CG (reprint author), Tongji Univ, Inst Prot Res, 1239 Siping Rd, Shanghai 200092, Peoples R China.,chunguangwang@mail.tongji.edu.cn
英文摘要	Conotoxins have been classified into several different superfamilies based on the highly conserved signal peptide sequences of their precursors. However, little is known about the five disulfide bonds containing S-superfamily conotoxins. Only two S-superfamily conotoxins have been identified but their cDNAs are not reported. In this work, we identified a novel S-superfamily conotoxin ca8a from vermivorous Conus caracteristicus. Its sequence shares no homology with those of two other previously reported toxins of the same superfamily, but they have the same cysteine framework, in particular the CX3CXC-CXC-CXCXC pattern at the C-terminal part. This implies that these toxins might have the same spatial scaffold, but different local conformation or residue side chains may be the cause of their different biological functions. Furthermore, the cDNA of ca8a was cloned with the RACE method. ca8a has a signal peptide sequence different from those of other conotoxins. This gives a defining feature of S-superfamily conotoxins and led to the cloning of more S-superfamily conotoxins from cone snails of different prey types, which indicates that S-superfamily conotoxins widely exist. These results will certainly enrich our understanding of the highly diversified S-superfamily conotoxins. (c) 2008 Elsevier Ltd. All rights reserved.
学科主题	Pharmacology & Pharmacy; Toxicology
类目[WOS]	Pharmacology & Pharmacy ; Toxicology
关键词[WOS]	DISULFIDE LINKAGE ; ION-CHANNEL ; PEPTIDES ; SUBTYPES ; VENOMS
收录类别	SCI
语种	英语
WOS记录号	WOS:000257645200002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1418]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liu, L,Wu, XC,Yuan, DD,et al. Identification of a novel S-superfamily conotoxin from vermivorous Conus caracteristicus[J]. TOXICON,2008,51(8):1331-1337.
APA	Liu, L,Wu, XC,Yuan, DD,Chi, CW,&Wang, CG.(2008).Identification of a novel S-superfamily conotoxin from vermivorous Conus caracteristicus.TOXICON,51(8),1331-1337.
MLA	Liu, L,et al."Identification of a novel S-superfamily conotoxin from vermivorous Conus caracteristicus".TOXICON 51.8(2008):1331-1337.