Oncolytic adenovirus-mediated shRNA against Apollon inhibits tumor cell growth and enhances antitumor effect of 5-fluorouracil

	Oncolytic adenovirus-mediated shRNA against Apollon inhibits tumor cell growth and enhances antitumor effect of 5-fluorouracil
	Chu, L; Gu, J; Sun, L; Qian, Q; Qian, C; Liu, X
刊名	GENE THERAPY
	2008
卷号	15 期号:7 页码:484-494
关键词	oncolytic adenovirus short hairpin RNA Apollon cancer therapy 5-fluorouracil
通讯作者	Liu, X (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,xyliu@sibs.ac.cn
英文摘要	Apollon, a membrane-associated inhibitor of apoptosis protein, protects cells against apoptosis and is upregulated in certain tumor cells. In this study, the effects of Apollon protein knockdown by RNA interference on the growth of human HeLa, HT-1080 and MCF-7 cells in vitro and in vivo were investigated. An oncolytic adenovirus (ZD55) containing the RNA polymerase III-dependent U6 promoter to express short hairpin RNA (shRNA) directed against Apollon (ZD55-siApollon) was constructed. Our data show that ZD55-siApollon successfully exerts a gene knockdown effect and causes the inhibition of tumor cell growth both in culture and in athymic mice in vivo. Cell cycle analysis, 4',6-diamidino-2-phenylindole staining and western blot analysis reveal that ZD55-siApollon-mediated suppression of Apollon induces apoptosis. Intratumoral injection of ZD55-siApollon significantly inhibits tumor growth in HT-1080 xenograft mice. Furthermore, ZD55-siApollon enhances the antitumor effect of 5-fluorouracil, a chemotherapeutic agent. In conclusion, these results suggest that the depletion of Apollon by oncolytic adenovirus-shRNA delivery system provides a promising method for cancer therapy.
学科主题	Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine
类目[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental
关键词[WOS]	E2/E3 UBIQUITIN LIGASE ; MAMMALIAN-CELLS ; CANCER-CELLS ; IN-VIVO ; HEPATOCELLULAR-CARCINOMA ; APOPTOSIS INHIBITORS ; RNA INTERFERENCE ; GENE-EXPRESSION ; IAP ; BRUCE
收录类别	SCI
语种	英语
WOS记录号	WOS:000254123500002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1407]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Chu, L,Gu, J,Sun, L,et al. Oncolytic adenovirus-mediated shRNA against Apollon inhibits tumor cell growth and enhances antitumor effect of 5-fluorouracil[J]. GENE THERAPY,2008,15(7):484-494.
APA	Chu, L,Gu, J,Sun, L,Qian, Q,Qian, C,&Liu, X.(2008).Oncolytic adenovirus-mediated shRNA against Apollon inhibits tumor cell growth and enhances antitumor effect of 5-fluorouracil.GENE THERAPY,15(7),484-494.
MLA	Chu, L,et al."Oncolytic adenovirus-mediated shRNA against Apollon inhibits tumor cell growth and enhances antitumor effect of 5-fluorouracil".GENE THERAPY 15.7(2008):484-494.