Caprin-2 enhances canonical Wnt signaling through regulating LRP5/6 phosphorylation

	Caprin-2 enhances canonical Wnt signaling through regulating LRP5/6 phosphorylation
	Ding, Y; Xi, Y; Chen, T; Wang, JY; Tao, DL; Wu, ZL; Li, YP; Li, C; Zeng, R; Li, L
刊名	JOURNAL OF CELL BIOLOGY
	2008
卷号	182 期号:5 页码:865-872
通讯作者	Li, L (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,lli@sibs.ac.cn
英文摘要	T he low-density lipoprotein receptor-related proteins 5 and 6 (LRP5/6) are coreceptors for Frizzled and transmit signals from the plasma membrane to the cytosol. However, the mechanism for LRP5/6 signal transmission remains undefined. Here, we identify cytoplasmic activation/proliferation-associated protein 2 (Caprin-2) as a LRP5/6-binding protein. Our data show that Caprin-2 stabilizes cytosolic beta-catenin and enhances lymphoid enhancer-binding factor 1/T cell factor-dependent reporter gene activity as well as the expression of Wnt target genes in mammalian cells. Morpholino-mediated knockdown of Caprin-2 in zebrafish embryos inhibits Wnt/beta-catenin signaling and results in a dorsalized phenotype. Moreover, Caprin-2 facilitates LRP5/6 phosphorylation by glycogen synthase kinase 3, and thus enhances the interaction between Axin and LRP5/6. Therefore, Caprin-2 promotes activation of the canonical Wnt signaling pathway by regulating LRP5/6 phosphorylation.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	BETA-CATENIN ; ZEBRAFISH ; PROTEINS ; LRP6 ; RECEPTOR ; INHIBITOR ; PATHWAY ; INTERNALIZATION ; TRANSDUCTION ; ACTIVATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000259050700007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1375]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Ding, Y,Xi, Y,Chen, T,et al. Caprin-2 enhances canonical Wnt signaling through regulating LRP5/6 phosphorylation[J]. JOURNAL OF CELL BIOLOGY,2008,182(5):865-872.
APA	Ding, Y.,Xi, Y.,Chen, T.,Wang, JY.,Tao, DL.,...&Li, L.(2008).Caprin-2 enhances canonical Wnt signaling through regulating LRP5/6 phosphorylation.JOURNAL OF CELL BIOLOGY,182(5),865-872.
MLA	Ding, Y,et al."Caprin-2 enhances canonical Wnt signaling through regulating LRP5/6 phosphorylation".JOURNAL OF CELL BIOLOGY 182.5(2008):865-872.