Mammalian DNMTs in the male germ line DNA of Drosophila

	Mammalian DNMTs in the male germ line DNA of Drosophila
	Weyrich, A; Tang, XJ; Xu, GL; Schrattenholz, A; Hunzinger, C; Hennig, W
刊名	BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE
	2008
卷号	86 期号:5 页码:380-385
关键词	Drosophila DNA methylation male germ line spermatogenesis DNMT3
通讯作者	Hennig, W (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, MPG Guest Lab, DAAD Lab, Shanghai 200031, Peoples R China.,whennig@gmx.de
英文摘要	It is controversial whether DNA methylation plays a functional role in Drosophila. We have studied testis DNA of Drosophila melanogaster Meigen, 1830 with antisera against 5-methylcytosine (5mC) and found no evidence for the presence of significant amounts of 5mC. Reactions occur only with I of 3 5mC antisera, but they are restricted to nuclear regions without detectable amounts of DNA. The antisera apparently cross-react with other nuclear components. If the murine de novo DNA methyltransferases, DNMT3A and DNMT3B, are expressed under the control of the spermatocyte-specific beta 2-tubulin promoter in testes, DNA methylation is not increased and no effects on the fertility of the fly are seen. DNA methylation has, therefore, no functional relevance in the male germ line of Drosophila.
学科主题	Biochemistry & Molecular Biology; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	CYTOSINE-5 METHYLTRANSFERASES ; Y-CHROMOSOME ; DE-NOVO ; METHYLATION ; MELANOGASTER ; SEQUENCES ; SPERMATOGENESIS ; IDENTIFICATION ; EXPRESSION ; PROTEINS
收录类别	SCI
语种	英语
WOS记录号	WOS:000262711600003
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1373]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Weyrich, A,Tang, XJ,Xu, GL,et al. Mammalian DNMTs in the male germ line DNA of Drosophila[J]. BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE,2008,86(5):380-385.
APA	Weyrich, A,Tang, XJ,Xu, GL,Schrattenholz, A,Hunzinger, C,&Hennig, W.(2008).Mammalian DNMTs in the male germ line DNA of Drosophila.BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE,86(5),380-385.
MLA	Weyrich, A,et al."Mammalian DNMTs in the male germ line DNA of Drosophila".BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE 86.5(2008):380-385.