Dopamine transporter inhibitory and antiparkinsonian effect of common flowering quince extract

	Dopamine transporter inhibitory and antiparkinsonian effect of common flowering quince extract
	Zhao, G; Jiang, ZH; Zheng, XW; Zang, SY; Guo, LH
刊名	PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR
	2008
卷号	90 期号:3 页码:363-371
关键词	common flowering quince Parkinson's disease antiparkinsonism dopamine reuptake inhibitor transporter
通讯作者	Guo, LH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,lhguo@sibs.ac.cn
英文摘要	Common flowering quince (FQ) is the fruit of Chaenomeles speciosa (Sweet) Nakai. FQ-containing cocktails have been applied to the treatment of neuralgia, migraine, and depression in traditional Chinese medicine. The present study assessed whether FQ is effective in dopamine transporter (DAT) regulation and antiparkinsonism by utilizing in vitro and in vivo assays, respectively. FQ at concentrations of 1-1000 mu g/ml concentration-dependently inhibited dopamine uptake by Chinese hamster ovary (CHO) cells stably expressing DAT (D8 cells) and by synaptorsomes. FQ had a slight inhibitory action on norepinephrine uptake by CHO cells expressing the norepinephrine transporter and no inhibitory effect on gamma-aminobutyric acid (GABA) uptake by CHO cells expressing GABA transporter-1 or serotonin uptake by the serotonin transporter. A viability assay showed that FQ mitigated 1-methyl-4-phenylpyridinium-induced toxicity in D8 cells. Furthermore, in behavioral studies, FQ alleviated rotational behavior in 6-hydroxydopamine-treated rats and improved deficits in endurance performance in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)treated mice. Furthermore, immunohistochemistry revealed that FQ markedly reduced the loss of tyrosine hydroxylase-positive neurons in the substantia nigra in MPTP-treated mice. In summary, FQ is a selective, potent DAT inhibitor and has antiparkinsonian-like effects that are mediated possibly by DAT suppression. FQ has the potential to be further developed for Parkinson's disease treatment. (C) 2008 Elsevier Inc. All rights reserved.
学科主题	Behavioral Sciences; Neurosciences & Neurology; Pharmacology & Pharmacy
类目[WOS]	Behavioral Sciences ; Neurosciences ; Pharmacology & Pharmacy
关键词[WOS]	MPTP-TREATED PRIMATES ; PARKINSONS-DISEASE ; CHAENOMELES-SPECIOSA ; REUPTAKE INHIBITION ; MOLECULAR PATHWAYS ; MECHANISMS ; DYSKINESIA ; SEROTONIN ; LEVODOPA ; AGONISTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000257362700011
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1353]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhao, G,Jiang, ZH,Zheng, XW,et al. Dopamine transporter inhibitory and antiparkinsonian effect of common flowering quince extract[J]. PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR,2008,90(3):363-371.
APA	Zhao, G,Jiang, ZH,Zheng, XW,Zang, SY,&Guo, LH.(2008).Dopamine transporter inhibitory and antiparkinsonian effect of common flowering quince extract.PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR,90(3),363-371.
MLA	Zhao, G,et al."Dopamine transporter inhibitory and antiparkinsonian effect of common flowering quince extract".PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR 90.3(2008):363-371.