XIAP-associated factor 1 (XAF1) suppresses angiogenesis in mouse endothelial cells

	XIAP-associated factor 1 (XAF1) suppresses angiogenesis in mouse endothelial cells
	Qiao, L; Gu, Q; Dai, Y; Shen, Z; Liu, XY; Qi, R; Ma, J; Zou, B; Lia, ZS; Lan, HY
刊名	TUMOR BIOLOGY
	2008
卷号	29 期号:2 页码:122-129
关键词	XAF1 angiogenesis endothelial cells apoptosis
通讯作者	Wong, BCY (reprint author), Univ Hong Kong, Queen Mary Hosp, Dept Med, Hong Kong, Hong Kong, Peoples R China.,bcywong@hku.hk
英文摘要	Objectives: XIAP-associated factor 1 (XAF1) is a tumor suppressor gene, but its role in angiogenesis is unknown. We investigated whether XAF1 has any antiangiogenesis effect. Methods: MS1 (a mouse endothelial cell line) was infected with an adenoviral vector ZD55-XAF1. Controls were uninfected or infected with ZD55-EGFP. Wound healing assay and tube formation assay were used to assess angiogenesis. Cell proliferation was detected by WST-1 assay, and apoptosis was detected by TUNEL and APOPercentage (TM) assays. Results: ZD55-XAF1 significantly upregulated XAF1, which was associated with decreased cell proliferation, migration and tube formation of MS1 cells. Ectopic overexpression of XAF1 induced apoptosis in MS1 and also sensitized cells to 5-fluorouracil-induced apoptosis. A significant decrease in the expression of Tie-1, Ang-1, Ang-2 and c-Myc was observed. Conclusions: Our data suggest that XAF1 possesses a potential antiangiogenesis effect. Suppressed expression of Tie-1, Ang-1, Ang-2 and c-Myc may be mechanistically responsible for the observed antiangiogenesis effect. Copyright (C) 2008 S. Karger AG, Basel.
学科主题	Oncology
类目[WOS]	Oncology
关键词[WOS]	GROWTH-FACTOR ; C-MYC ; SURVIVAL FACTOR ; EXPRESSION ; ANGIOPOIETIN-1 ; ANTAGONIST ; APOPTOSIS ; MECHANISMS ; RECEPTOR ; TIE2
收录类别	SCI
语种	英语
WOS记录号	WOS:000257903500007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1351]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Qiao, L,Gu, Q,Dai, Y,et al. XIAP-associated factor 1 (XAF1) suppresses angiogenesis in mouse endothelial cells[J]. TUMOR BIOLOGY,2008,29(2):122-129.
APA	Qiao, L.,Gu, Q.,Dai, Y.,Shen, Z.,Liu, XY.,...&Wong, BCY.(2008).XIAP-associated factor 1 (XAF1) suppresses angiogenesis in mouse endothelial cells.TUMOR BIOLOGY,29(2),122-129.
MLA	Qiao, L,et al."XIAP-associated factor 1 (XAF1) suppresses angiogenesis in mouse endothelial cells".TUMOR BIOLOGY 29.2(2008):122-129.