Insulin analogs with B24 or B25 phenylalanine replaced by biphenylalanine

	Insulin analogs with B24 or B25 phenylalanine replaced by biphenylalanine
	Du, HJ; Shi, JH; Cui, DF; Zhang, YS
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2008
卷号	40 期号:2 页码:133-139
关键词	insulin biphenylalanine circular dichroism fluoremetry self-association
通讯作者	Zhang, YS (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,yszhang@sibs.ac.cn
英文摘要	B24 and B25 phenylalanines (Phe) play important roles in insulin structure and function. Insulin analogs with B24 Phe or B25 Phe replaced by biphenylalanine (Bip) were prepared by enzymatic semisynthesis. The biological activities were determined by receptor binding assay and in vivo mouse convulsion assay. The results showed that B25 Bip insulin has 139% receptor binding activity and 50% in vivo biological activity, whereas B24 Bip insulin is inactive, when compared with native insulin, suggesting that B24 Phe is crucial for insulin activity. The structures in solution were studied by circular dichroism and fluoremetry, and our results suggested that the insulin analogs with low activities tend to be more tightly packed. The association properties were studied by size exclusion chromatography. The Bip-amide replacement of B24 Phe in deshexapeptide insulin or B25 Phe in despentapeptide insulin will cause the monomeric B24 Phe-amide deshexapeptide insulin or B25 Phe-amide despentapeptide insulin to associate and form dimers, whereas the mutations of B24 Phe in insulin will make insulin dimers dissociate into insulin monomers.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	CIRCULAR-DICHROISM ; SIDE-CHAIN ; RECEPTOR ; CONFORMATION ; RESIDUES ; BINDING ; BOVINE
收录类别	SCI
语种	英语
WOS记录号	WOS:000253501900006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1337]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Du, HJ,Shi, JH,Cui, DF,et al. Insulin analogs with B24 or B25 phenylalanine replaced by biphenylalanine[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2008,40(2):133-139.
APA	Du, HJ,Shi, JH,Cui, DF,&Zhang, YS.(2008).Insulin analogs with B24 or B25 phenylalanine replaced by biphenylalanine.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,40(2),133-139.
MLA	Du, HJ,et al."Insulin analogs with B24 or B25 phenylalanine replaced by biphenylalanine".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 40.2(2008):133-139.