Regulation of Phosphatidylinositol Kinases and Metabolism by Wnt3a and Dvl

	Regulation of Phosphatidylinositol Kinases and Metabolism by Wnt3a and Dvl
	Qin, YB; Li, L; Pan, WJ; Wu, DQ
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2009
卷号	284 期号:34 页码:22544-22548
通讯作者	Pan, WJ (reprint author), Yale Univ, Sch Med, Vasc Biol & Therapeut Program, 333 Cedar St, New Haven, CT 06520 USA.,weijun.pan@yale.edu ; dan.wu@yale.edu
英文摘要	Wnt signaling plays important roles in various physiological and pathophysiological processes. The pathway that leads to beta-catenin stabilization is initiated by Wnt binding to its cell surface receptors, which induces the formation of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P(2)) via activation of phosphatidylinositol 4-phosphate 5-kinase (PIP5K) type I. Here, we show that Wnt also stimulated the production of phosphatidylinositol 4-phosphate (PtdIns(4)P), which depended on Frizzled (Fz), Dishevelled (Dvl), and phosphatidylinositol 4-kinase (PI4K) type II alpha in HEK293T cells. Dvl directly interacted with and activated PI4KII alpha by increasing its V(max) for ATP and PtdIns. In addition, Dvl regulated PI4KII alpha and PIP5KI via different domains. Moreover, Dvl, PI4KII alpha, and PIP5KI appeared to form a ternary complex upon Wnt3a stimulation. This complex may allow efficient production of PtdIns(4,5)P(2) from PtdIns, which is far more abundant than PtdIns(4)P in cells. Therefore, this study provides new insights into the mechanism by which Wnt3a regulates the production of PtdIns(4,5)P(2).
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	LRP6 PHOSPHORYLATION ; SIGNALING PATHWAY ; DISEASE ; ACTIVATION ; PHOSPHOINOSITIDES ; MECHANISM ; AXIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000268963100007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1291]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Qin, YB,Li, L,Pan, WJ,et al. Regulation of Phosphatidylinositol Kinases and Metabolism by Wnt3a and Dvl[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2009,284(34):22544-22548.
APA	Qin, YB,Li, L,Pan, WJ,&Wu, DQ.(2009).Regulation of Phosphatidylinositol Kinases and Metabolism by Wnt3a and Dvl.JOURNAL OF BIOLOGICAL CHEMISTRY,284(34),22544-22548.
MLA	Qin, YB,et al."Regulation of Phosphatidylinositol Kinases and Metabolism by Wnt3a and Dvl".JOURNAL OF BIOLOGICAL CHEMISTRY 284.34(2009):22544-22548.