Macropinocytosis contributes to the macrophage foam cell formation in RAW264.7 cells

	Macropinocytosis contributes to the macrophage foam cell formation in RAW264.7 cells
	Yao, WQ; Li, K; Liao, K
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2009
卷号	41 期号:9 页码:773-780
关键词	macrophage macropinocytosis foam cell PI3-kinase atherosclerosis
通讯作者	Liao, K (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,kliao@sibs.ac.cn
英文摘要	The key event in the atherosclerosis development is the lipids uptake by macrophage and the formation of foam cell in subendothelial arterial space. Besides the uptake of modified low-density lipoprotein (LDL) by scavenger receptor-mediated endocytosis, macrophages possess constitutive macropinocytosis, which is capable of taking up a large quantity of solute. Macrophage foam cell formation could be induced in RAW264.7 cells by increasing the serum concentration in the culture medium. Foam cell formation induced by serum could be blocked by phosphoinositide 3-kinase inhibitor, LY294002 or wortmannin, which inhibited macropinocytosis but not receptor-mediated endocytosis. Further analysis indicated that macropinocytosis took place at the gangliosides-enriched membrane area. Cholesterol depletion by beta-methylcyclodextrin-blocked macropinocytosis without affecting scavenger receptor-mediated endocytosis of modified LDLs. These results suggested that macropinocytosis might be one of the important mechanisms for lipid uptake in macrophage. And it made significant contribution to the lipid accumulation and foam cell formation.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	LOW-DENSITY-LIPOPROTEIN ; COLONY-STIMULATING FACTOR ; SCAVENGER RECEPTOR-A ; GROWTH-FACTOR ; IN-VITRO ; M-CSF ; ATHEROSCLEROSIS ; MICE ; MEMBRANE ; PATHWAYS
收录类别	SCI
语种	英语
WOS记录号	WOS:000269959500007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1234]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yao, WQ,Li, K,Liao, K. Macropinocytosis contributes to the macrophage foam cell formation in RAW264.7 cells[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2009,41(9):773-780.
APA	Yao, WQ,Li, K,&Liao, K.(2009).Macropinocytosis contributes to the macrophage foam cell formation in RAW264.7 cells.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,41(9),773-780.
MLA	Yao, WQ,et al."Macropinocytosis contributes to the macrophage foam cell formation in RAW264.7 cells".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 41.9(2009):773-780.