Functional analysis of ScSwi1 and CaSwi1 in invasive and pseudohyphal growth of Saccharomyces cerevisiae

	Functional analysis of ScSwi1 and CaSwi1 in invasive and pseudohyphal growth of Saccharomyces cerevisiae
	Mao, XM; Nie, XY; Cao, F; Chen, JY
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2009
卷号	41 期号:7 页码:594-602
关键词	ScSwi1 CaSwi1 invasive growth pseudohyphal growth FLO11 Saccharomyces cerevisiae
通讯作者	Chen, JY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,jychen@sibs.ac.cn
英文摘要	Here we reported that, in Saccharomyces cerevisiae, deleting Swi1 (ScSwi1), a core component in Swi/Snf complex, caused defects of invasive growth, pseudohyphal growth, FLO11 expression, and proper cell separation. Re-introduction of SWI1 into the swi1 mutants could suppress all defects observed. We also showed that overproducing Swi1 could suppress the defect of flo8 cells in pseudohyphal growth in diploids, but not invasive growth in haploids. Overexpression of SWI1 could not bypass the requirement of Ste12 or Tec1 in invasive growth or pseudohyphal growth. We concluded that the Swi/Snf complex was required for FLO11 expression and proper cell separation, and both the FLO8 and STE12 genes should be present for the complex to function for the invasive growth but only the STE12 gene was required for the pseudohyphal growth. Ectopic expression of Candida albicans SWI1 (CaSWI1) could partially complement the defects examined of haploid Scswi1 mutants, but failed to complement the defects examined of diploid Scswi1/Scswi1 mutants. Overexpressing CaSwi1 mitigated invasive and pseudohyphal growth defects resulting from deletions in the MAP kinase and cAMP pathways. The integrity of S. cerevisiae Swi/Snf complex is required for invasive and filamentous growth promoted by overexpressing CaSwi1.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	CANDIDA-ALBICANS ; FILAMENTOUS GROWTH ; SWI/SNF COMPLEX ; MAP KINASE ; YEAST ; EXPRESSION ; GENE ; ACTIVATION ; SWI1 ; RECRUITMENT
收录类别	SCI
语种	英语
WOS记录号	WOS:000268889000008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1218]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Mao, XM,Nie, XY,Cao, F,et al. Functional analysis of ScSwi1 and CaSwi1 in invasive and pseudohyphal growth of Saccharomyces cerevisiae[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2009,41(7):594-602.
APA	Mao, XM,Nie, XY,Cao, F,&Chen, JY.(2009).Functional analysis of ScSwi1 and CaSwi1 in invasive and pseudohyphal growth of Saccharomyces cerevisiae.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,41(7),594-602.
MLA	Mao, XM,et al."Functional analysis of ScSwi1 and CaSwi1 in invasive and pseudohyphal growth of Saccharomyces cerevisiae".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 41.7(2009):594-602.