Mannose-exposing myeloid leukemia cells detected by the sCAR-PPA fusion protein

	Mannose-exposing myeloid leukemia cells detected by the sCAR-PPA fusion protein
	Li, GC; Li, N; Zhang, YH; Li, X; Wang, YG; Liu, XY; Qian, WB; Liu, XC
刊名	INTERNATIONAL JOURNAL OF HEMATOLOGY
	2009
卷号	89 期号:5 页码:611-617
关键词	Mannose Myeloid leukemia Pinellia pedatisecta agglutinin Adenovirus
通讯作者	Qian, WB (reprint author), Zhejiang Univ, Coll Med, Dept Hematol, Affiliated Hosp 1, Hangzhou 310003, Zhejiang, Peoples R China.,qianwenb@yahoo.com.cn ; xcliu@zstu.edu.cn
英文摘要	Altered glycosylation may be a hallmark of malignant transformation and cancer progression. In the work described, a specific mannose-binding lectin, Pinellia pedatisecta agglutinin (PPA), was genetically fused with the extracellular domain of coxsackie-adenovirus receptor (CAR) to generate the soluble CAR (sCAR)-PPA fusion protein. The adenoviral transduction of acute myeloid leukemia (AML) cell lines Kasumi-1 and HL-60 was increased by sCAR-PPA, indicating that a fraction of AML cells exposing mannose residues was detected by PPA. However, sCAR-PPA did not increase the adenoviral infection of KG-1 cells, suggesting the mannose exposure of AML cells may be cell type specific. Furthermore, the infectious efficiency of Ad-EGFP in chronic myeloid leukemia cell line K562 was significantly increased by sCAR-PPA as well. We, herein, report that PPA recognized a fraction of myeloid leukemia cells showing mannose-exposing phenotype. The sCAR-PPA fusion protein combined with the adenoviral vector system may provide a useful tool for investigating myeloid leukemia cells exposing mannose residues and further elucidating the role of these cells in the leukemia development.
学科主题	Hematology
类目[WOS]	Hematology
关键词[WOS]	ACUTE LYMPHOBLASTIC-LEUKEMIA ; N-ACETYLGLUCOSAMINYLTRANSFERASE-V ; COLON-CANCER CELLS ; BINDING LECTIN ; PINELLIA-PEDATISECTA ; ADENOVIRUS VECTOR ; EXPRESSION ; CHILDHOOD ; ADHESION ; GLYCANS
收录类别	SCI
语种	英语
WOS记录号	WOS:000266583200008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1190]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, GC,Li, N,Zhang, YH,et al. Mannose-exposing myeloid leukemia cells detected by the sCAR-PPA fusion protein[J]. INTERNATIONAL JOURNAL OF HEMATOLOGY,2009,89(5):611-617.
APA	Li, GC.,Li, N.,Zhang, YH.,Li, X.,Wang, YG.,...&Liu, XC.(2009).Mannose-exposing myeloid leukemia cells detected by the sCAR-PPA fusion protein.INTERNATIONAL JOURNAL OF HEMATOLOGY,89(5),611-617.
MLA	Li, GC,et al."Mannose-exposing myeloid leukemia cells detected by the sCAR-PPA fusion protein".INTERNATIONAL JOURNAL OF HEMATOLOGY 89.5(2009):611-617.