Nudel and FAK as Antagonizing Strength Modulators of Nascent Adhesions through Paxillin

	Nudel and FAK as Antagonizing Strength Modulators of Nascent Adhesions through Paxillin
	Shan, YL; Yu, LH; Li, Y; Pan, YD; Zhang, QG; Wang, FB; Chen, JF; Zhu, XL
刊名	PLOS BIOLOGY
	2009
卷号	7 期号:5 页码:e1000116-e1000116
通讯作者	Shan, YL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai, Peoples R China.,xlzhu@sibs.ac.cn
英文摘要	Adhesion and detachment are coordinated critical steps during cell migration. Conceptually, efficient migration requires both effective stabilization of membrane protrusions at the leading edge via nascent adhesions and their successful persistence during retraction of the trailing side via disruption of focal adhesions. As nascent adhesions are much smaller in size than focal adhesions, they are expected to exhibit a stronger adhesivity in order to achieve the coordination between cell front and back. Here, we show that Nudel knockdown by interference RNA (RNAi) resulted in cell edge shrinkage due to poor adhesions of membrane protrusions. Nudel bound to paxillin, a scaffold protein of focal contacts, and colocalized with it in areas of active membrane protrusions, presumably at nascent adhesions. The Nudel-paxillin interaction was disrupted by focal adhesion kinase (FAK) in a paxillin-binding-dependent manner. Forced localization of Nudel in all focal contacts by fusing it to paxillin markedly strengthened their adhesivity, whereas overexpression of structurally activated FAK or any paxillin-binding FAK mutant lacking the N-terminal autoinhibitory domain caused cell edge shrinkage. These results suggest a novel mechanism for selective reinforcement of nascent adhesions via interplays of Nudel and FAK with paxillin to facilitate cell migration.
学科主题	Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics
类目[WOS]	Biochemistry & Molecular Biology ; Biology
关键词[WOS]	NEURONAL MIGRATION DEFECTS ; EARLY EMBRYONIC LETHALITY ; CELL-MIGRATION ; CYTOPLASMIC DYNEIN ; FOCAL ADHESIONS ; LEADING-EDGE ; REGULATES ADHESION ; RHO-GTPASES ; TGF-BETA ; PROTEIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000267282000014
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1186]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Shan, YL,Yu, LH,Li, Y,et al. Nudel and FAK as Antagonizing Strength Modulators of Nascent Adhesions through Paxillin[J]. PLOS BIOLOGY,2009,7(5):e1000116-e1000116.
APA	Shan, YL.,Yu, LH.,Li, Y.,Pan, YD.,Zhang, QG.,...&Zhu, XL.(2009).Nudel and FAK as Antagonizing Strength Modulators of Nascent Adhesions through Paxillin.PLOS BIOLOGY,7(5),e1000116-e1000116.
MLA	Shan, YL,et al."Nudel and FAK as Antagonizing Strength Modulators of Nascent Adhesions through Paxillin".PLOS BIOLOGY 7.5(2009):e1000116-e1000116.