The differential protein and lipid compositions of noncaveolar lipid microdomains and caveolae

	The differential protein and lipid compositions of noncaveolar lipid microdomains and caveolae
	Yao, Y; Hong, SY; Zhou, H; Yuan, TC; Zeng, R; Liao, K
刊名	CELL RESEARCH
	2009
卷号	19 期号:4 页码:497-506
关键词	membrane lipid microdomains caveolae noncaveolar lipid microdomains lipid rafts sucrose density gradient sodium carbonate extraction
通讯作者	Liao, K (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,kliao@sibs.ac.cn
英文摘要	Morphologically, caveolae and lipid rafts are two different membrane structures. They are often reported to share similar lipid and protein compositions, and are considered to be two subtypes of membrane lipid microdomains. By modifying sucrose density gradient flotation centrifugation, which is used to isolate lipid microdomains, we were able to separate caveolae and noncaveolar lipid microdomains into two distinct fractions. The caveolar membranes are membrane vesicles of 100-nm diameter, enriched with caveolin-1 and flotillin-1. The noncaveolar lipid microdomains are amorphous membranes and most likely the coalescence of heterogeneous lipid rafts. They are depleted of caveolin-1 and are more enriched with cholesterol and sphingolipids than the caveolae. Many membrane proteins, such as insulin-like growth factor-1 receptor (membrane receptor), aquaporin-1 (membrane transporter), Thy-1 and N-cadherin (glycosylphosphatidylinositol-anchored membrane protein and membrane glycoprotein), are specifically associated with noncaveolar lipid microdomains, but not with caveolae. These results indicate that the lipid and protein compositions of caveolae differ from those of noncaveolar lipid microdomains. The difference in their protein compositions implies that these two membrane microdomains may have different cellular functions.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	SIGNAL-TRANSDUCING MOLECULES ; INSULIN-RECEPTOR SUBSTRATE-1 ; THIN-LAYER CHROMATOGRAPHY ; CHOLESTEROL DEPLETION ; PLASMA-MEMBRANE ; CELL-MEMBRANES ; T-CADHERIN ; RAFTS ; DOMAINS ; ADIPOCYTES
收录类别	SCI
语种	英语
WOS记录号	WOS:000265700100011
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1179]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yao, Y,Hong, SY,Zhou, H,et al. The differential protein and lipid compositions of noncaveolar lipid microdomains and caveolae[J]. CELL RESEARCH,2009,19(4):497-506.
APA	Yao, Y,Hong, SY,Zhou, H,Yuan, TC,Zeng, R,&Liao, K.(2009).The differential protein and lipid compositions of noncaveolar lipid microdomains and caveolae.CELL RESEARCH,19(4),497-506.
MLA	Yao, Y,et al."The differential protein and lipid compositions of noncaveolar lipid microdomains and caveolae".CELL RESEARCH 19.4(2009):497-506.