Improvement of Morphine-Mediated Analgesia by Inhibition of beta-Arrestin 2 Expression in Mice Periaqueductal Gray Matter

	Improvement of Morphine-Mediated Analgesia by Inhibition of beta-Arrestin 2 Expression in Mice Periaqueductal Gray Matter
	Li, YT; Liu, X; Liu, C; Kang, JH; Yang, JY; Pei, G; Wu, CF
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2009
卷号	10 期号:3 页码:954-963
关键词	beta-Arrestin 2 Lentivirus Periaqueductal gray Analgesia Morphine
通讯作者	Wu, CF (reprint author), Shenyang Pharmaceut Univ, Dept Pharmacol, Shenyang 110016, Peoples R China.,genggeng800816@yahoo.com ; xingliu@fudan.edu.cn ; lonelyrunner27@yahoo.com.cn ; jhkang@sibs.ac.cn ; yangjingyu2006@gmail.com ; gpei@sibs.ac.cn ; wucf@syphu.edu.cn
英文摘要	Morphine is a well-known mu-opioid receptor (MOR) agonist and an efficient analgesic, but its long-term use inevitably leads to drug addiction and tolerance. Here, we show that specific inhibition of mu-arrestin2 with its siRNA lentivirus microinjected in mice periaqueductal gray matter (PAG) significantly improved both acute and chronic morphine analgesia and delayed the tolerance in the hotplate test. The specific effect of beta-arrestin2 was proven by overexpression or knockdown of its homology beta-arrestin1 in PAG, which showed no significant effects on morphine analgesia. These findings suggest that specific siRNA targeting beta-arrestin2 may constitute a new approach to morphine therapy and other MOR agonist-mediated analgesia and tolerance.
学科主题	Biochemistry & Molecular Biology; Chemistry
类目[WOS]	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
关键词[WOS]	COUPLED RECEPTOR KINASES ; MU-OPIOID RECEPTORS ; KNOCK-OUT MICE ; RAT-BRAIN ; BETA-ARRESTIN-2 ; TOLERANCE ; DESENSITIZATION ; GENE ; EFFICACY ; LIGANDS
收录类别	SCI
语种	英语
WOS记录号	WOS:000264565300014
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1165]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, YT,Liu, X,Liu, C,et al. Improvement of Morphine-Mediated Analgesia by Inhibition of beta-Arrestin 2 Expression in Mice Periaqueductal Gray Matter[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2009,10(3):954-963.
APA	Li, YT.,Liu, X.,Liu, C.,Kang, JH.,Yang, JY.,...&Wu, CF.(2009).Improvement of Morphine-Mediated Analgesia by Inhibition of beta-Arrestin 2 Expression in Mice Periaqueductal Gray Matter.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,10(3),954-963.
MLA	Li, YT,et al."Improvement of Morphine-Mediated Analgesia by Inhibition of beta-Arrestin 2 Expression in Mice Periaqueductal Gray Matter".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 10.3(2009):954-963.