Identification, expression, and characterization of the highly conserved D-xylose isomerase in animals

	Identification, expression, and characterization of the highly conserved D-xylose isomerase in animals
	Ding, M; Teng, Y; Yin, QY; Chen, W; Zhao, FK
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2009
卷号	41 期号:2 页码:116-122
关键词	xylose isomerase xylanase animals D-xylose Ciona intestinalis heterogeneous expression
通讯作者	Zhao, FK (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Prote, Shanghai 20031, Peoples R China.,fkzhao@sibs.ac.cn
英文摘要	D-xylose is a necessary sugar for animals. The xylanase from a mollusk, Ampullaria crossean, was previously reported by our laboratory. This xylanase can degrade the xylan into D-xylose. But there is still a gap in our knowledge on its metabolic pathway. The question is how does the xylose enter the pentose pathway? With the help of genomic databases and bioinformatic tools, we found that some animals, such as bacteria, have a highly conserved D-xylose isomerase (EC 5.3.1.5). The xylose isomerase from a sea squirt, Ciona intestinalis, was heterogeneously expressed in Escherichia coli and purified to confirm its function. The recombinant enzyme had good thermal stability in the presence of Mg(2+). At the optimum temperature and optimum pH environment, its specific activity on D-xylose was 0.331 mu mol/mg/min. This enzyme exists broadly in many animals, but it disappeared in the genome of Amphibia-like Xenopus laevis. Its sequence was highly conserved. The xylose isomerases from animals are very interesting proteins for the study of evolution.
学科主题	Biochemistry & Molecular Biology ; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	ORIGIN ; METABOLISM ; GENOMICS ; ENZYMES ; GENES
收录类别	SCI
语种	英语
WOS记录号	WOS:000263834300003
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1147]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Ding, M,Teng, Y,Yin, QY,et al. Identification, expression, and characterization of the highly conserved D-xylose isomerase in animals[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2009,41(2):116-122.
APA	Ding, M,Teng, Y,Yin, QY,Chen, W,&Zhao, FK.(2009).Identification, expression, and characterization of the highly conserved D-xylose isomerase in animals.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,41(2),116-122.
MLA	Ding, M,et al."Identification, expression, and characterization of the highly conserved D-xylose isomerase in animals".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 41.2(2009):116-122.