A double-regulated oncolytic adenovirus with improved safety for adenocarcinoma therapy

	A double-regulated oncolytic adenovirus with improved safety for adenocarcinoma therapy
	Wei, N; Fan, JK; Gu, JF; He, LF; Tang, WH; Cao, X; Liu, XY
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2009
卷号	388 期号:2 页码:234-239
关键词	Oncolytic adenovirus MUC1 promoter Safety Replication specificity
通讯作者	Liu, XY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China.,xyliu@sibs.ac.cn
英文摘要	Safety and efficiency are equally important to be considered in developing oncolytic adenovirus. Previously, we have reported that ZD55, an oncolytic adenovirus with the deletion of E1B-55K gene, exhibited potent antitumor activity. In this study, to improve the safety of ZD55, we utilized MUC1 promoter to replace the native promoter of E1A on the basis of ZD55, and generated a double-regulated adenovirus, named MUD55. Our data demonstrated that the expression of early and late genes of MUD55 was both reduced in MUC1-negative cells, resulting in its stricter glandular-tumor selective progeny production. The cytopathic effect of MUD55 was about 10-fold lower than mono-regulated adenovirus ZD55 or Ad. MUC1 in normal cells and not obviously attenuated in glandular tumor cells. Moreover, MUD55 showed the least liver toxicity when administrated by intravenous injection in nude mice. These results indicate that MUD55 could be a promising candidate for the treatment of adenocarcinoma. (C) 2009 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	REPLICATION-COMPETENT ADENOVIRUS ; POTENT ANTITUMORAL EFFICACY ; BREAST-CARCINOMA CELLS ; TUMOR-CELLS ; GENE-EXPRESSION ; CANCER-CELLS ; ONYX-015 ; PROTEIN ; SELECTIVITY ; PROMOTER
收录类别	SCI
语种	英语
WOS记录号	WOS:000274534300011
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1140]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wei, N,Fan, JK,Gu, JF,et al. A double-regulated oncolytic adenovirus with improved safety for adenocarcinoma therapy[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2009,388(2):234-239.
APA	Wei, N.,Fan, JK.,Gu, JF.,He, LF.,Tang, WH.,...&Liu, XY.(2009).A double-regulated oncolytic adenovirus with improved safety for adenocarcinoma therapy.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,388(2),234-239.
MLA	Wei, N,et al."A double-regulated oncolytic adenovirus with improved safety for adenocarcinoma therapy".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 388.2(2009):234-239.