Potential application of alternatively glycosylated serum MUC1 and MUC5AC in gastric cancer diagnosis

	Potential application of alternatively glycosylated serum MUC1 and MUC5AC in gastric cancer diagnosis
	Xu, Y; Zhang, L; Hu, GX
刊名	BIOLOGICALS
	2009
卷号	37 期号:1 页码:18-25
关键词	Gastric cancer Glycosylation Mucins Tumor marker Cancer diagnosis
通讯作者	Hu, GX (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yueyang Rd, Shanghai 20031, Peoples R China.,hgxgene@sunm.shcnc.ac.cn
英文摘要	Post-transcription modification of proteins can be altered during carcinogenesis. In this study, quantitative sandwich enzyme immunoassays were utilized to explore the clinical diagnostic value of the alternatively glycosylated MUC1 and MUC5AC. Four pairs of antibodies were selected to construct quantitative sandwich enzyme immunoassay. Serum mucin levels of 104 primary gastric cancer patients and 120 healthy individuals were measured using the four antibody pairs. The detection sensitivities of each antibody pair against gastric cancers were 42.31%, 25.00%, 38.46% and 30.77% respectively, with a specificity of 90.00%, significantly higher than widely used tumor markers CEA (21.15%) and CA19-9 (18.27%). When monitoring in parallel with all of the four antibody pairs, the detection sensitivity increased to 75.00%, with the same 90.00% specificity. Immunoblotting of the serum samples using the anti-mucin antibodies revealed highly variable glycosylation patterns among gastric cancer patients. In addition, real-time PCR indicated the elevated mRNA levels of MUC1 and/or MUC5AC in gastric cancers. The cancer-specific epitopes were also detected in other alimentary canal epithelium cancers such as colonic, nasopharyngeal and esophageal cancers, but with much lower sensitivities. Our results suggested that alternatively glycosylated MUC1 and MUC5AC could be of significant potential as effective tumor markers in gastric cancer diagnosis. (c) 2008 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Pharmacology & Pharmacy
类目[WOS]	Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Pharmacology & Pharmacy
关键词[WOS]	MONOCLONAL-ANTIBODIES ; GENE-EXPRESSION ; ALTERED GLYCOSYLATION ; BREAST-CANCER ; CORE PROTEIN ; CARCINOMAS ; CELLS ; RNA ; CHOLANGIOCARCINOMA ; EPITHELIUM
收录类别	SCI
语种	英语
WOS记录号	WOS:000263167700003
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1126]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xu, Y,Zhang, L,Hu, GX. Potential application of alternatively glycosylated serum MUC1 and MUC5AC in gastric cancer diagnosis[J]. BIOLOGICALS,2009,37(1):18-25.
APA	Xu, Y,Zhang, L,&Hu, GX.(2009).Potential application of alternatively glycosylated serum MUC1 and MUC5AC in gastric cancer diagnosis.BIOLOGICALS,37(1),18-25.
MLA	Xu, Y,et al."Potential application of alternatively glycosylated serum MUC1 and MUC5AC in gastric cancer diagnosis".BIOLOGICALS 37.1(2009):18-25.