Generation of Pig Induced Pluripotent Stem Cells with a Drug-Inducible System

	Generation of Pig Induced Pluripotent Stem Cells with a Drug-Inducible System
	Wu, Z; Chen, JJ; Ren, JT; Bao, L; Liao, J; Cui, C; Rao, LJ; Li, H; Gu, YJ; Dai, HM
刊名	JOURNAL OF MOLECULAR CELL BIOLOGY
	2009
卷号	1 期号:1 页码:46-54
关键词	pluripotency reprogram iPS cells pig ungulate embryonic stem cells
通讯作者	Xiao, L (reprint author), Chinese Acad Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, Cell Bank,Stem Cell Bank,Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,leixiao@sibs.ac.cn
英文摘要	Domesticated ungulate pluripotent embryonic stem (ES) cell lines would be useful for generating precise gene-modified animals. To date, many efforts have been made to establish domesticated ungulate pluripotent ES cells from early embryos without success. Here, we report the generation of porcine-induced pluripotent stem (iPS) cells using drug-inducible expression of defined factors. We showed that porcine iPS cells expressed alkaline phosphatase, SSEA3, SSEA4, Tra-1-60, Tra-1-81, Oct3/4, Nanog, Sox2, Rex1 and CDH1. Pig iPS cells expressed high levels of telomerase activity and showed normal karyotypes. These cells could differentiate into cell types of all three germ layers in vitro and in teratomas. Our study reveals properties of porcine pluripotent stem cells that may facilitate the eventual establishment of porcine ES cells. Moreover, the porcine iPS cells produced may be directly useful for the generation of precise gene-modified pigs.
学科主题	Cell Biology
类目[WOS]	Cell Biology
收录类别	SCI
语种	英语
WOS记录号	WOS:000207813000011
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1116]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wu, Z,Chen, JJ,Ren, JT,et al. Generation of Pig Induced Pluripotent Stem Cells with a Drug-Inducible System[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2009,1(1):46-54.
APA	Wu, Z.,Chen, JJ.,Ren, JT.,Bao, L.,Liao, J.,...&Xiao, L.(2009).Generation of Pig Induced Pluripotent Stem Cells with a Drug-Inducible System.JOURNAL OF MOLECULAR CELL BIOLOGY,1(1),46-54.
MLA	Wu, Z,et al."Generation of Pig Induced Pluripotent Stem Cells with a Drug-Inducible System".JOURNAL OF MOLECULAR CELL BIOLOGY 1.1(2009):46-54.