beta-Glucan Oligosaccharide Enhances CD8(+) TCells Immune Response Induced by a DNA Vaccine Encoding Hepatitis B Virus Core Antigen

	beta-Glucan Oligosaccharide Enhances CD8(+) TCells Immune Response Induced by a DNA Vaccine Encoding Hepatitis B Virus Core Antigen
	Wang, J; Dong, SF; Liu, CH; Wang, W; Sun, SH; Gu, JX; Wang, YA; Boraschi, D; Qu, D
刊名	JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY
	2010
期号	1 页码:645213-645213
通讯作者	Qu, D (reprint author), Fudan Univ, Shanghai Med Coll, Inst Med Microbiol, Key Lab Med Mol Virol,Minist Educ & Hlth, Shanghai 200032, Peoples R China.,dqu@shmu.edu.cn
英文摘要	DNA vaccination can induce specific CD8(+) T cell immune response, but the response level is low in large mammals and human beings. Coadministration of an adjuvant can optimize protective immunity elicited by a DNA vaccine. In this study, we investigated the effect of a synthetic glucohexaose (beta-glu6), an analogue of Lentinan basic unit, on specific CD8(+) T cell response induced by a DNA vaccine encoding HBcAg (pB144) in mice. We found that beta-glu6 promoted the recruitment and maturation of dendritic cells, enhanced the activation of CD8(+) and CD4(+) T cells and increased the number of specific CD8(+)/IFN-gamma(+) T cells in lymphoid and nonlymphoid tissues in mice immunized by pB144. Immunization with pB144 and beta-glu6 increased the anti-HBc IgG and IgG2a antibody titer. These results demonstrate that beta-glu6 can enhance the virus-specific CTL and Th1 responses induced by DNA vaccine, suggesting beta-glu6 as a candidate adjuvant in DNA vaccination.
学科主题	Biotechnology & Applied Microbiology; Research & Experimental Medicine
类目[WOS]	Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
关键词[WOS]	DENDRITIC CELLS ; T-CELLS ; ANTITUMOR-ACTIVITY ; LENTINAN ; IMMUNIZATION ; PROTEIN ; GENE ; BOND ; POLYSACCHARIDE ; COIMMUNIZATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000279791900001
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1108]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, J,Dong, SF,Liu, CH,et al. beta-Glucan Oligosaccharide Enhances CD8(+) TCells Immune Response Induced by a DNA Vaccine Encoding Hepatitis B Virus Core Antigen[J]. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY,2010(1):645213-645213.
APA	Wang, J.,Dong, SF.,Liu, CH.,Wang, W.,Sun, SH.,...&Qu, D.(2010).beta-Glucan Oligosaccharide Enhances CD8(+) TCells Immune Response Induced by a DNA Vaccine Encoding Hepatitis B Virus Core Antigen.JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY(1),645213-645213.
MLA	Wang, J,et al."beta-Glucan Oligosaccharide Enhances CD8(+) TCells Immune Response Induced by a DNA Vaccine Encoding Hepatitis B Virus Core Antigen".JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY .1(2010):645213-645213.