Platelet Endothelial Cell Adhesion Molecule-1, Stage-Specific Embryonic Antigen-1, and Flk-1 Mark Distinct Populations of Mouse Embryonic Stem Cells During Differentiation Toward Hematopoietic/Endothelial Cells

	Platelet Endothelial Cell Adhesion Molecule-1, Stage-Specific Embryonic Antigen-1, and Flk-1 Mark Distinct Populations of Mouse Embryonic Stem Cells During Differentiation Toward Hematopoietic/Endothelial Cells
	Yue, W; Pi, QM; Zhang, WJ; Zhou, GD; Cui, L; Liu, W; Cao, YL
刊名	STEM CELLS AND DEVELOPMENT
	2010
卷号	19 期号:12 页码:1937-1948
通讯作者	Zhang, WJ (reprint author), Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Natl Tissue Engn Ctr China, Dept Plast & Reconstruct Surg,Sch Med, 639 Zhi Zao Ju Rd, Shanghai 200011, Peoples R China.,wenjieboshi@yahoo.com.cn ; yilincao@yahoo.com
英文摘要	Vascular endothelial cells (ECs) and most hematopoietic cells express platelet endothelial cell adhesion molecule-1 (PECAM-1), which is the cell surface protein also expressed in mouse embryonic stem (ES) cells. To better understand how PECAM-1(+) ES cells differentiate into PECAM-1(+) hematopoietic cells/ECs, 3 cell surface markers, PECAM-1, stage-specific embryonic antigen-1(SSEA-1), and Flk-1, were utilized to dissect the developmental process during ES cell differentiation in vitro. Undifferentiated ES cells expressed PECAM-1, with a majority of them coexpressing SSEA-1. During ES cell differentiation, expression of PECAM-1 decreased to give rise to PECAM-1(-)/SSEA-1(+) cells, which represented epiblast stem cells. Subsequently, Flk-1-expressing cells developed from PECAM-1(-)/SSEA-1(+) cells, becoming SSEA-1(-)/Flk-1(+) through the downregulation of SSEA-1 expression. Following this, a second wave of PECAM-1 expression, which represented the mature hematopoietic cells/ECs, developed from Flk-1(+) cells. Also, a small portion of PECAM-1(+)/SSEA-1(+) cells, which represented the residual undifferentiated ES cells, were consistently observed in long-term differentiated embryoid bodies. This work revealed a sequential change in PECAM-1, SSEA-1, and Flk-1 expression during ES cell differentiation; therefore, they could be valuable cell surface markers for isolating cells at distinct developmental stages in ES cell differentiation.
学科主题	Cell Biology; Hematology; Research & Experimental Medicine; Transplantation
类目[WOS]	Cell & Tissue Engineering ; Hematology ; Medicine, Research & Experimental ; Transplantation
关键词[WOS]	IN-VITRO DIFFERENTIATION ; CD31-DEFICIENT MICE ; LINEAGE ANALYSIS ; EXPRESSION ; CULTURE ; PRECURSORS ; EPIBLAST ; PECAM-1 ; VASCULOGENESIS ; HEMANGIOBLAST
收录类别	SCI
语种	英语
WOS记录号	WOS:000284626400011
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1081]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yue, W,Pi, QM,Zhang, WJ,et al. Platelet Endothelial Cell Adhesion Molecule-1, Stage-Specific Embryonic Antigen-1, and Flk-1 Mark Distinct Populations of Mouse Embryonic Stem Cells During Differentiation Toward Hematopoietic/Endothelial Cells[J]. STEM CELLS AND DEVELOPMENT,2010,19(12):1937-1948.
APA	Yue, W.,Pi, QM.,Zhang, WJ.,Zhou, GD.,Cui, L.,...&Cao, YL.(2010).Platelet Endothelial Cell Adhesion Molecule-1, Stage-Specific Embryonic Antigen-1, and Flk-1 Mark Distinct Populations of Mouse Embryonic Stem Cells During Differentiation Toward Hematopoietic/Endothelial Cells.STEM CELLS AND DEVELOPMENT,19(12),1937-1948.
MLA	Yue, W,et al."Platelet Endothelial Cell Adhesion Molecule-1, Stage-Specific Embryonic Antigen-1, and Flk-1 Mark Distinct Populations of Mouse Embryonic Stem Cells During Differentiation Toward Hematopoietic/Endothelial Cells".STEM CELLS AND DEVELOPMENT 19.12(2010):1937-1948.