PHF8 is a histone H3K9me2 demethylase regulating rRNA synthesis

	PHF8 is a histone H3K9me2 demethylase regulating rRNA synthesis
	Zhu, ZQ; Wang, YR; Li, X; Wang, YQ; Xu, LY; Wang, X; Sun, TL; Dong, XB; Chen, LL; Mao, HL
刊名	CELL RESEARCH
	2010
卷号	20 期号:7 页码:794-801
关键词	PHF8 histone demethylase H3K9me2 rRNA synthesis
通讯作者	Chen, PA (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai Key Lab Mol Androl, 310 Yueyang Rd, Shanghai 100031, Peoples R China.,pinchen@sibs.ac.cn ; cdchen@sibs.ac.cn
英文摘要	Dimethylation of histone H3 lysine 9 (H3K9me2) is an important epigenetic mark associated with transcription repression. Here, we identified PHF8, a JmjC-domain-containing protein, as a histone demethylase specific for this repressing mark. Recombinant full-length wild type protein could remove methylation from H3K9me2, but mutation of a conserved histidine to alanine H247A abolished the demethylase activity. Overexpressed exogenous PHF8 was colocalized with B23 staining. Endogenous PHF8 was also colocalized with B23 and fibrillarin, two well-established nucleolus proteins, suggesting that PHF8 is localized in the nucleolus and may regulate rRNA transcription. Indeed, PHF8 bound to the promoter region of the rDNA gene. Knockdown of PHF8 reduced the expression of rRNA, and overexpression of the gene resulted in upregulation of rRNA transcript. Concomitantly, H3K9me2 level was elevated in the promoter region of the rDNA gene in PHF8 knockdown cells and reduced significantly when the wild type but not the catalytically inactive H247A mutant PHF8 was overexpressed. Thus, our study identified a histone demethylase for H3K9me2 that regulates rRNA transcription.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	DOMAIN-CONTAINING PROTEINS ; STRUCTURAL INSIGHTS ; LYSINE DEMETHYLASE ; METHYLATION ; METHYLTRANSFERASE ; CHROMATIN ; GENES ; G9A ; TRANSCRIPTION ; DIMETHYL
收录类别	SCI
语种	英语
WOS记录号	WOS:000280079500008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1029]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhu, ZQ,Wang, YR,Li, X,et al. PHF8 is a histone H3K9me2 demethylase regulating rRNA synthesis[J]. CELL RESEARCH,2010,20(7):794-801.
APA	Zhu, ZQ.,Wang, YR.,Li, X.,Wang, YQ.,Xu, LY.,...&Chen, CD.(2010).PHF8 is a histone H3K9me2 demethylase regulating rRNA synthesis.CELL RESEARCH,20(7),794-801.
MLA	Zhu, ZQ,et al."PHF8 is a histone H3K9me2 demethylase regulating rRNA synthesis".CELL RESEARCH 20.7(2010):794-801.