p300-Mediated Acetylation Stabilizes the Th-Inducing POK Factor

	p300-Mediated Acetylation Stabilizes the Th-Inducing POK Factor
	Zhang, M; Zhang, JL; Rui, JX; Liu, XL
刊名	JOURNAL OF IMMUNOLOGY
	2010
卷号	185 期号:7 页码:3960-3969
通讯作者	Liu, XL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,liux@sibs.ac.cn
英文摘要	The lineage-specifying factor Th-inducing POK (ThPOK) directs the intrathymic differentiation of CD4 T cells. Although the regulation of ThPOK at the transcription level has been extensively studied, specific posttranslational modifications regulating the activity of ThPOK have not been addressed. In this paper, we show that ThPOK is an unstable protein that is more readily degraded in CD8 T cells compared with CD4 T cells. Among the various proteins that bind ThPOK, acetyltransferase p300 specifically promotes the acetylation of ThPOK at K210, K216, and K339, outcompeting ubiquitination, thereby stabilizing the protein. In CD4 T cells, attenuation of p300-mediated acetylation promotes the degradation of ThPOK. In contrast, mutation of lysines 210, 216, and 339 to arginines stabilizes ThPOK and enhances its ability to suppress the expression of CD8 molecule and cytotoxic effectors in CD8 T cells. Our results reveal an essential role of p300-mediated acetylation in regulating the stability of ThPOK and suggest that such regulation may play a part in CD4/CD8 lineage differentiation. The Journal of Immunology, 2010, 185: 3960-3969.
学科主题	Immunology
类目[WOS]	Immunology
关键词[WOS]	CD4-CD8 LINEAGE COMMITMENT ; POSITIVE SELECTION ; CELL DEVELOPMENT ; NONHISTONE PROTEINS ; THPOK ; DIFFERENTIATION ; RUNX ; UBIQUITINATION ; PHOSPHORYLATION ; EXPRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000282059500022
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1025]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, M,Zhang, JL,Rui, JX,et al. p300-Mediated Acetylation Stabilizes the Th-Inducing POK Factor[J]. JOURNAL OF IMMUNOLOGY,2010,185(7):3960-3969.
APA	Zhang, M,Zhang, JL,Rui, JX,&Liu, XL.(2010).p300-Mediated Acetylation Stabilizes the Th-Inducing POK Factor.JOURNAL OF IMMUNOLOGY,185(7),3960-3969.
MLA	Zhang, M,et al."p300-Mediated Acetylation Stabilizes the Th-Inducing POK Factor".JOURNAL OF IMMUNOLOGY 185.7(2010):3960-3969.