Treatment of intracerebral haemorrhage in rats with intraventricular transplantation of human amniotic epithelial cells

	Treatment of intracerebral haemorrhage in rats with intraventricular transplantation of human amniotic epithelial cells
	Dong, WL; Chen, HY; Yang, XX; Guo, LH; Hui, GZ
刊名	CELL BIOLOGY INTERNATIONAL
	2010
卷号	34 期号:6 页码:573-577
关键词	cerebral haemorrhage epithelial cell rat transplantation
通讯作者	Dong, WL (reprint author), Soochow Univ, Dept Neurol, Affiliated Hosp 1, Suzhou 215006, Peoples R China.,dwlsz@163.com
英文摘要	We explored the effects on brain oedema and neurological functional recovery after transplantation of hAECs (human amniotic epithelial cells) into the lateral ventricle of rats with ICH (intracerebral haemorrhage). hAECs were isolated from human term placenta and seeded for primary culture. We delivered hAECs labelled with Hoechst33258 and transfected with EGFP (enhanced green fluorescent protein) gene using lentiviral vectors into ICH rat models. The behaviour of the animals and brain oedema were evaluated after 28 days, and brain sections were made for morphological and immunohistochemical analyses with fluorescence microscopy. Our results were as follows. Transplanted hAECs were observed along the lateral wall and survived for at least 4 weeks. Some of the cells were stained with human specific antibody to vimentin and nestin. Around the injury site, activated microglia stained with OX42 were reduced. The water content of ICH rats decreased in the treatment group. The behaviour test scores were improved in the treatment group compared with those in the control groups. In conclusion, hAECs cannot only survive in the lateral ventricle of ICH rats after transplantation, but also express vimentin and nestin. hAEC transplantation reduced brain oedema and improved the motor deficits of ICH rats.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	BRAIN-INJURY ; EXPRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000278793500002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1016]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Dong, WL,Chen, HY,Yang, XX,et al. Treatment of intracerebral haemorrhage in rats with intraventricular transplantation of human amniotic epithelial cells[J]. CELL BIOLOGY INTERNATIONAL,2010,34(6):573-577.
APA	Dong, WL,Chen, HY,Yang, XX,Guo, LH,&Hui, GZ.(2010).Treatment of intracerebral haemorrhage in rats with intraventricular transplantation of human amniotic epithelial cells.CELL BIOLOGY INTERNATIONAL,34(6),573-577.
MLA	Dong, WL,et al."Treatment of intracerebral haemorrhage in rats with intraventricular transplantation of human amniotic epithelial cells".CELL BIOLOGY INTERNATIONAL 34.6(2010):573-577.