Runx2 regulates survivin expression in prostate cancer cells

	Runx2 regulates survivin expression in prostate cancer cells
	Lim, M; Zhong, C; Yang, SX; Bell, AM; Cohen, MB; Roy-Burman, P
刊名	LABORATORY INVESTIGATION
	2010
卷号	90 期号:2 页码:222-233
关键词	bone morphogenetic protein-7 survivin Runx2 apoptosis prostate cancer
通讯作者	Roy-Burman, P (reprint author), Univ So Calif, Keck Sch Med, Dept Pathol, 2011 Zonal Ave, Los Angeles, CA 90033 USA.,royburma@usc.edu
英文摘要	Previously we described that bone morphogenetic protein-7 (BMP7) could protect prostate cancer C4-2B cells from serum starvation-induced apoptosis via survivin induction. Here, for the first time, we identify Runx2 as a key regulator of survivin transcription. In C4-2B cells grown normally, suppression of Runx2 reduced survivin expression. Using ChIP assays, two regions of the survivin promoter, -1953 to -1812 (I) and -1485 to -1119 (II) encompassing consensus Runx-binding sites were examined. Runx2 was found to be associated with both regions, with a stronger affinity to region-I. In serum-starved cells neither region was occupied, but BMP7 restored association to region-II and not region-I. In reporter assays, transcription activity by BMP7 was significantly reduced when sequences including binding sites of region-II were deleted. Additionally, Runx2 expression was enhanced by BMP7 in these cells. Along with a strong survivin expression, a trend in increased Runx2 expression in human prostate cancer cells and tissues was noted. In the conditional Pten-knockout mouse, Runx2 level increased with growth of prostate tumor. The data define a novel role of Runx2 in regulating survivin expression in malignant epithelial cells and identify it as a critical factor in BMP signaling that protects cancer cells against apoptosis. Laboratory Investigation (2010) 90, 222-233; doi:10.1038/labinvest.2009.128; published online 30 November 2009
学科主题	Research & Experimental Medicine; Pathology
类目[WOS]	Medicine, Research & Experimental ; Pathology
关键词[WOS]	BONE MORPHOGENETIC PROTEIN-7 ; METASTATIC PROSTATE ; OSTEOBLAST DIFFERENTIATION ; TRANSCRIPTIONAL REGULATION ; GENE-EXPRESSION ; BREAST-CANCER ; MOUSE MODELS ; SMAD ; IDENTIFICATION ; ACTIVATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000274103700007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/959]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Lim, M,Zhong, C,Yang, SX,et al. Runx2 regulates survivin expression in prostate cancer cells[J]. LABORATORY INVESTIGATION,2010,90(2):222-233.
APA	Lim, M,Zhong, C,Yang, SX,Bell, AM,Cohen, MB,&Roy-Burman, P.(2010).Runx2 regulates survivin expression in prostate cancer cells.LABORATORY INVESTIGATION,90(2),222-233.
MLA	Lim, M,et al."Runx2 regulates survivin expression in prostate cancer cells".LABORATORY INVESTIGATION 90.2(2010):222-233.