The serine/threonine kinase LKB1 controls thymocyte survival through regulation of AMPK activation and Bcl-XL expression

	The serine/threonine kinase LKB1 controls thymocyte survival through regulation of AMPK activation and Bcl-XL expression
	Cao, YH; Li, H; Liu, HF; Zheng, C; Ji, HB; Liu, XL
刊名	CELL RESEARCH
	2010
卷号	20 期号:1 页码:99-108
关键词	LKB1 AMPK Bcl-XL thymocyte survival development
通讯作者	Liu, XL (reprint author), Chinese Acad Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,liux@sibs.ac.cn
英文摘要	LKB1 is a serine/threonine kinase that directly activates the energy sensor AMP-activated protein kinase (AMPK) in response to bioenergetic stress, and mainly acts as a tumor suppressor that controls cell polarity and proliferation. Although LKB1 is expressed in multiple tissues including the thymus and the spleen, its roles in T-cell development and function remain unknown. Here, we show that T-cell-specific deletion of LKB1 resulted in reduced survival of double-positive (DP) thymocytes and impaired generation of both CD4 and CD8 single-positive thymocytes. Disruption of LKB1 not only prevented the activation of AMPK but also impaired the expression of anti-apoptotic protein Bcl-XL. Importantly, ectopic expression of either Bcl-XL or the constitutively active AMPK mutant significantly rescued DP thymocytes from LKB1 deficiency-induced cell death. Moreover, ectopic expression of the constitutively active AMPK mutant was found to restore the expression of Bcl-XL in LKB1-deficient DP thymocytes. These findings identify LKB1 as a critical factor for the survival of DP thymocytes through regulation of AMPK activation and Bcl-XL expression.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	T-CELL-RECEPTOR ; PROTEIN-KINASE ; TUMOR-SUPPRESSOR ; POSITIVE SELECTION ; ANTIGEN RECEPTOR ; BETA-SELECTION ; TCR SIGNALS ; ROR-GAMMA ; METABOLISM ; GENE
收录类别	SCI
语种	英语
WOS记录号	WOS:000273790500011
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/930]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Cao, YH,Li, H,Liu, HF,et al. The serine/threonine kinase LKB1 controls thymocyte survival through regulation of AMPK activation and Bcl-XL expression[J]. CELL RESEARCH,2010,20(1):99-108.
APA	Cao, YH,Li, H,Liu, HF,Zheng, C,Ji, HB,&Liu, XL.(2010).The serine/threonine kinase LKB1 controls thymocyte survival through regulation of AMPK activation and Bcl-XL expression.CELL RESEARCH,20(1),99-108.
MLA	Cao, YH,et al."The serine/threonine kinase LKB1 controls thymocyte survival through regulation of AMPK activation and Bcl-XL expression".CELL RESEARCH 20.1(2010):99-108.