Tet-Mediated Formation of 5-Carboxylcytosine and Its Excision by TDG in Mammalian DNA

	Tet-Mediated Formation of 5-Carboxylcytosine and Its Excision by TDG in Mammalian DNA
	He, YF; Li, BZ; Li, Z; Liu, P; Wang, Y; Tang, QY; Ding, JP; Jia, YY; Chen, ZC; Li, L
刊名	SCIENCE
	2011
卷号	333 期号:6047 页码:1303-1307
通讯作者	Xu, GL (reprint author), Chinese Acad Sci, Grp DNA Metab, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,glxu@sibs.ac.cn
英文摘要	The prevalent DNA modification in higher organisms is the methylation of cytosine to 5-methylcytosine (5mC), which is partially converted to 5-hydroxymethylcytosine (5hmC) by the Tet (ten eleven translocation) family of dioxygenases. Despite their importance in epigenetic regulation, it is unclear how these cytosine modifications are reversed. Here, we demonstrate that 5mC and 5hmC in DNA are oxidized to 5-carboxylcytosine (5caC) by Tet dioxygenases in vitro and in cultured cells. 5caC is specifically recognized and excised by thymine-DNA glycosylase (TDG). Depletion of TDG in mouse embyronic stem cells leads to accumulation of 5caC to a readily detectable level. These data suggest that oxidation of 5mC by Tet proteins followed by TDG-mediated base excision of 5caC constitutes a pathway for active DNA demethylation.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	EMBRYONIC STEM-CELLS ; GLYCOSYLASE ACTIVITY ; GENE-EXPRESSION ; 5-HYDROXYMETHYLCYTOSINE ; DEMETHYLATION ; METHYLATION ; REPAIR ; DEAMINATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000294406400058
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/894]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	He, YF,Li, BZ,Li, Z,et al. Tet-Mediated Formation of 5-Carboxylcytosine and Its Excision by TDG in Mammalian DNA[J]. SCIENCE,2011,333(6047):1303-1307.
APA	He, YF.,Li, BZ.,Li, Z.,Liu, P.,Wang, Y.,...&Xu, GL.(2011).Tet-Mediated Formation of 5-Carboxylcytosine and Its Excision by TDG in Mammalian DNA.SCIENCE,333(6047),1303-1307.
MLA	He, YF,et al."Tet-Mediated Formation of 5-Carboxylcytosine and Its Excision by TDG in Mammalian DNA".SCIENCE 333.6047(2011):1303-1307.