Role of tRNA amino acid-accepting end in aminoacylation and its quality control

	Role of tRNA amino acid-accepting end in aminoacylation and its quality control
	Zhou, XL; Du, DH; Tan, M; Lei, HY; Ruan, LL; Eriani, G; Wang, ED
刊名	NUCLEIC ACIDS RESEARCH
	2011
卷号	39 期号:20 页码:8857-8868
通讯作者	Wang, ED (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,edwang@sibs.ac.cn
英文摘要	Aminoacyl-tRNA synthetases (aaRSs) are remarkable enzymes that are in charge of the accurate recognition and ligation of amino acids and tRNA molecules. The greatest difficulty in accurate aminoacylation appears to be in discriminating between highly similar amino acids. To reduce mischarging of tRNAs by non-cognate amino acids, aaRSs have evolved an editing activity in a second active site to cleave the incorrect aminoacyl-tRNAs. Editing occurs after translocation of the aminoacyl-CCA(76) end to the editing site, switching between a hairpin and a helical conformation for aminoacylation and editing. Here, we studied the consequence of nucleotide changes in the CCA(76) accepting end of tRNA(Leu) during the aminoacylation and editing reactions. The analysis showed that the terminal A(76) is essential for both reactions, suggesting that critical interactions occur in the two catalytic sites. Substitutions of C(74) and C(75) selectively decreased aminoacylation keeping nearly unaffected editing. These mutations might favor the regular helical conformation required to reach the editing site. Mutating the editing domain residues that contribute to CCA(76) binding reduced the aminoacylation fidelity leading to cell-toxicity in the presence of non-cognate amino acids. Collectively, the data show how protein synthesis quality is controlled by the CCA(76) homogeneity of tRNAs.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	CCA-ADDING ENZYME ; LEUCYL-TRANSFER ; CRYSTAL-STRUCTURE ; AQUIFEX-AEOLICUS ; SYNTHETASE ; TRNA(LEU) ; RECOGNITION ; COMPLEX ; DOMAIN ; DISCRIMINATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000296343400024
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/881]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhou, XL,Du, DH,Tan, M,et al. Role of tRNA amino acid-accepting end in aminoacylation and its quality control[J]. NUCLEIC ACIDS RESEARCH,2011,39(20):8857-8868.
APA	Zhou, XL.,Du, DH.,Tan, M.,Lei, HY.,Ruan, LL.,...&Wang, ED.(2011).Role of tRNA amino acid-accepting end in aminoacylation and its quality control.NUCLEIC ACIDS RESEARCH,39(20),8857-8868.
MLA	Zhou, XL,et al."Role of tRNA amino acid-accepting end in aminoacylation and its quality control".NUCLEIC ACIDS RESEARCH 39.20(2011):8857-8868.