Intracerebroventricular transplantation of human amniotic epithelial cells ameliorates spatial memory deficit in the doubly transgenic mice coexpressing APPswe and PS1 Delta E9-deleted genes

	Intracerebroventricular transplantation of human amniotic epithelial cells ameliorates spatial memory deficit in the doubly transgenic mice coexpressing APPswe and PS1 Delta E9-deleted genes
	Xue, SR; Chen, CF; Dong, WL; Hui, GZ; Liu, TJ; Guo, LH
刊名	CHINESE MEDICAL JOURNAL
	2011
卷号	124 期号:17 页码:2642-2648
关键词	Alzheimer's disease human amniotic epithelial cells transgenic mice spatial memory deficit
通讯作者	Guo, LH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,huiguozhen@163.com ; guolihe@cellstar.com.cn
英文摘要	Background Human amniotic epithelial cells (HAECs), which have characteristics of both embryonic and pluripotent stem cells, are therefore a candidate in cell therapy without creating legal or ethical problems. In the present study, we aimed to investigate the effects of intracerebroventricular transplantation of HAECs on doubly transgenic mice of Alzheimer's disease (AD) coexpressing presenilin-1 (PS 1) and mutant Sweden amyloid precursor protein (APPswe) genes. Methods The offspring mice genotypes were detected using PCR identification of APPswe and PS1 gene. The doubly transgenic (TG) mice (n=20) and wild-type (WT) mice (n=20) were randomly divided into two groups respectively: the transplantation group treated with HAECs and the control group with phosphate buffered saline. Six radial arm water maze test was used to assess the spatial memory in the TG and WT mice. Amyloid plaques and neurofibrillary tangles were analyzed using congo red and acid-silver methenamine staining respectively. Immunofluorescence cytochennistry was used to track the survival of HAECs. Immunohistochemistry was used to determine the expression of octamer-binding protein 4 (Oct-4) and Nanog in the HAECs. High performance liquid chromatography was used to measure acetylcholine in hippocampus. The density of cholinergic neurons in basal forebrain and nerve fibers in hippocampus was measured using acetylcholinesterase staining. Results Amyloid deposition occurred in hippocampus and frontal cortex in the double TG mice aged 8 months, but not in WT mice. The results also showed that transplanted HAECs can survive for at least 8 weeks and migrate to the third ventricle without immune rejection. The graft HAECs can also express the specific marker Oct-4 and Nanog of stem cell. Compared with the control group, transplantation of HAECs can not only significantly improve the spatial memory of the TG mice, but also increase acetylcholine concentration and the number of hippocampal cholinergic neurites. Conclusions These results demonstrate that intracerebroventricular transplantation of HAECs can improve the spatial memory of the double TG mice. The higher content of acetylcholine in hippocampus released by more survived cholinergic neurites is one of the causes of this improvement. Chin Med J 2011;124(17):2642-2648
学科主题	General & Internal Medicine
类目[WOS]	Medicine, General & Internal
关键词[WOS]	ALZHEIMERS-DISEASE ; MOUSE MODEL ; STEM-CELLS ; DIFFERENTIATION ; DYSFUNCTION ; EXPRESSION ; HYPOTHESIS ; THERAPY ; PROMOTE ; NEURONS
收录类别	SCI
语种	英语
WOS记录号	WOS:000295297400014
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/878]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xue, SR,Chen, CF,Dong, WL,et al. Intracerebroventricular transplantation of human amniotic epithelial cells ameliorates spatial memory deficit in the doubly transgenic mice coexpressing APPswe and PS1 Delta E9-deleted genes[J]. CHINESE MEDICAL JOURNAL,2011,124(17):2642-2648.
APA	Xue, SR,Chen, CF,Dong, WL,Hui, GZ,Liu, TJ,&Guo, LH.(2011).Intracerebroventricular transplantation of human amniotic epithelial cells ameliorates spatial memory deficit in the doubly transgenic mice coexpressing APPswe and PS1 Delta E9-deleted genes.CHINESE MEDICAL JOURNAL,124(17),2642-2648.
MLA	Xue, SR,et al."Intracerebroventricular transplantation of human amniotic epithelial cells ameliorates spatial memory deficit in the doubly transgenic mice coexpressing APPswe and PS1 Delta E9-deleted genes".CHINESE MEDICAL JOURNAL 124.17(2011):2642-2648.