Identification of MicroRNAs and Application of RNA Interference for Gene Targeting In Vivo in the Rat Epididymis

	Identification of MicroRNAs and Application of RNA Interference for Gene Targeting In Vivo in the Rat Epididymis
	Zhang, YL; Zhang, JS; Zhou, YC; Zhao, Y; Ni, MJ
刊名	JOURNAL OF ANDROLOGY
	2011
卷号	32 期号:6 页码:587-591
关键词	RNAi microRNA sperm maturation DEFB SERPIN LCN
通讯作者	Zhang, YL (reprint author), 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,ylzhang@sibs.ac.cn
英文摘要	Identification, detection, and use of small-ribonucleic acid (RNA) molecules have been paid increasing attention in the past decades. Here we show studies of two interrelated lines of this research in a sperm maturation-related organ, the epididymis. First, by using microarray and small-RNA library screening systems, a series of spatially and temporally regulated known and novel small regulatory RNA molecules, referred to as microRNAs, has been identified and characterized. Second, small double-stranded RNAs, called small interfering RNAs, emerged as a powerful tool to knock down gene expression by a pathway known as RNA interference. It has been successfully used to silence epididymal gene expression in vivo to elucidate the function of several sperm motility- and capacitation-related proteins in the rat epididymis.
学科主题	Endocrinology & Metabolism
类目[WOS]	Andrology
关键词[WOS]	SPERM MATURATION ; BETA-DEFENSIN ; FAMILY ; THERAPEUTICS ; EXPRESSION ; PROTEINS
收录类别	SCI
语种	英语
WOS记录号	WOS:000296037400005
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/819]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, YL,Zhang, JS,Zhou, YC,et al. Identification of MicroRNAs and Application of RNA Interference for Gene Targeting In Vivo in the Rat Epididymis[J]. JOURNAL OF ANDROLOGY,2011,32(6):587-591.
APA	Zhang, YL,Zhang, JS,Zhou, YC,Zhao, Y,&Ni, MJ.(2011).Identification of MicroRNAs and Application of RNA Interference for Gene Targeting In Vivo in the Rat Epididymis.JOURNAL OF ANDROLOGY,32(6),587-591.
MLA	Zhang, YL,et al."Identification of MicroRNAs and Application of RNA Interference for Gene Targeting In Vivo in the Rat Epididymis".JOURNAL OF ANDROLOGY 32.6(2011):587-591.