Defects in Trophoblast Cell Lineage Account for the Impaired In Vivo Development of Cloned Embryos Generated by Somatic Nuclear Transfer

	Defects in Trophoblast Cell Lineage Account for the Impaired In Vivo Development of Cloned Embryos Generated by Somatic Nuclear Transfer
	Lin, JW; Shi, LY; Zhang, M; Yang, H; Qin, YR; Zhang, J; Gong, DQ; Zhang, X; Li, DS; Li, JS
刊名	CELL STEM CELL
	2011
卷号	8 期号:4 页码:371-375
通讯作者	Li, JS (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,jsli@sibs.ac.cn
英文摘要	The low success rate of somatic nuclear transfer (NT) is hypothesized to be mainly due to functional defects in the trophoblast cell lineage rather than the inner cell mass (ICM); this hypothesis, however, remains to be tested directly. Here we separated the ICMs from cloned blastocysts and aggregated the cloned ICM with two fertilization-derived (FD) tetraploid (4N) embryos. We found that the full-term development of cloned ICMs was dramatically improved after the trophoblast cells in the cloned blastocysts were replaced by cells from tetraploid embryos, thus providing direct evidence that defects in trophoblast cell lineage underlie the low success rate of somatic NT.
学科主题	Cell Biology
类目[WOS]	Cell & Tissue Engineering ; Cell Biology
关键词[WOS]	STEM-CELLS ; ES CELLS ; MOUSE BLASTOCYSTS ; TETRAPLOID CELLS ; MICE
收录类别	SCI
语种	英语
WOS记录号	WOS:000289707100009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/774]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Lin, JW,Shi, LY,Zhang, M,et al. Defects in Trophoblast Cell Lineage Account for the Impaired In Vivo Development of Cloned Embryos Generated by Somatic Nuclear Transfer[J]. CELL STEM CELL,2011,8(4):371-375.
APA	Lin, JW.,Shi, LY.,Zhang, M.,Yang, H.,Qin, YR.,...&Li, JS.(2011).Defects in Trophoblast Cell Lineage Account for the Impaired In Vivo Development of Cloned Embryos Generated by Somatic Nuclear Transfer.CELL STEM CELL,8(4),371-375.
MLA	Lin, JW,et al."Defects in Trophoblast Cell Lineage Account for the Impaired In Vivo Development of Cloned Embryos Generated by Somatic Nuclear Transfer".CELL STEM CELL 8.4(2011):371-375.