Biosynthesis and distribution of Lewis X- and Lewis Y-containing glycoproteins in the murine male reproductive system

	Biosynthesis and distribution of Lewis X- and Lewis Y-containing glycoproteins in the murine male reproductive system
	Wang, CM; Huang, CY; Gu, YH; Zhou, YC; Zhu, ZM; Zhang, YL
刊名	GLYCOBIOLOGY
	2011
卷号	21 期号:2 页码:225-234
关键词	fucosyltransferase glycoproteins Lewis X Lewis Y male reproductive system
通讯作者	Zhou, YC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai Key Lab Mol Androl,State Key Lab Mol Bio, Shanghai, Peoples R China.,zhouych@sibs.ac.cn ; ylzhang@sibs.ac.cn
英文摘要	Although Lewis X (LeX) and Lewis Y (LeY) antigens were thought to play important roles in fertility, fucosyltransferase (Fut)-deficient (Fut1, Fut2 and Fut4) mice which lack LeX or LeY antigen are still fertile. In the present study, the Fut-deficient and wild-type mice were used to measure the expression of Fat mRNA along the mouse male reproductive tract and determine the role of each Fut in the biosynthesis of LeX/LeY antigens, which are conjugated to glycoproteins in the male reproductive system. LeX/LeY-containing glycoproteins were detected in the epididymis, vas deferens, seminal vesicle and coagulating gland, but not in the testis. We demonstrate that the synthesis of LeY-containing glycoproteins in the epididymis and vas deferens is catalyzed by Full and Fut4. In the seminal vesicle and the coagulating gland, they are mainly synthesized by Fut2 and an alpha-(1,3)-Fut, but not Fut4. The synthesis of LeX-containing glycoproteins in the middle caput epididymis is catalyzed by Fut4 and by Fut4 and Fut2 in the seminal vesicle. We provide evidence that LeX is synthesized in the coagulating gland by Fut9. We found that the lack of activity by one Fut does not completely inhibit LeX/LeY antigen expression in the male reproductive tract. This redundancy may help to explain why in vivo studies with Fat-deficient mice do not support the presumption that LeX/LeY antigens play important roles in male fertility. We provide details regarding the phenotypes of established Fut-deficient mice and lay the foundation for elucidating the functions of LeX/LeY antigens in other aspects of the male reproductive system.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	ALPHA-1,3-FUCOSYL-TRANSFERASE-IX FUT9 ; ALPHA(1,2)FUCOSYLTRANSFERASE GENES ; MOLECULAR-CLONING ; EXPRESSION ; MOUSE ; MICE ; TRACT ; EPIDIDYMIS ; ALPHA-1,2-FUCOSYL-TRANSFERASE ; IMPLANTATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000286717500009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/746]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, CM,Huang, CY,Gu, YH,et al. Biosynthesis and distribution of Lewis X- and Lewis Y-containing glycoproteins in the murine male reproductive system[J]. GLYCOBIOLOGY,2011,21(2):225-234.
APA	Wang, CM,Huang, CY,Gu, YH,Zhou, YC,Zhu, ZM,&Zhang, YL.(2011).Biosynthesis and distribution of Lewis X- and Lewis Y-containing glycoproteins in the murine male reproductive system.GLYCOBIOLOGY,21(2),225-234.
MLA	Wang, CM,et al."Biosynthesis and distribution of Lewis X- and Lewis Y-containing glycoproteins in the murine male reproductive system".GLYCOBIOLOGY 21.2(2011):225-234.