Expression and Characterization of Full-Length Ampullaria crossean Endoglucanase EG65s and Their Two Functional Modules

	Expression and Characterization of Full-Length Ampullaria crossean Endoglucanase EG65s and Their Two Functional Modules
	Yin, QY; Teng, YG; Li, YH; Ding, M; Zhao, FK
刊名	BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
	2011
卷号	75 期号:2 页码:240-246
关键词	endoglucanase EG65 carbohydrate-binding module adsorption isotherm Ampullaria crossean
通讯作者	Zhao, FK (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,fkzhao@sibs.ac.cn
英文摘要	Three endoglucanase cDNAs, eg65a, eg65b, and eg65c, were cloned from the mollusk Ampullaria crossean in previous work. To characterize the full-length enzymes as well as their individual functional modules via heterologous expression analysis, the three full-length putative endoglucanases (rEG65a, rEG65b, and rEG65c) and the corresponding catalytic modules (EG65a-CM, EG65b-CM, and EG65c-CM) were expressed in Pichia pastoris GS115, and the three corresponding carbohydrate-binding modules (EG65a-CBM, EG65b-CBM, and EG65c-CBM) were expressed in Escherichia coli BL21 (DE3). The properties of recombinant rEG65b, EG65a-CM, EG65b-CM, and EG65c-CM were characterized. Binding assays of CBMs with insoluble polysaccharides indicated that both EG65b-CBM and EG65c-CBM bound to phosphoric-acid swollen cellulose (PASC), Avicel, and oat-spelt xylan, while EG65a-CBM did not. The relative equilibrium constants (K-r) of EG65b-CBM and EG65c-CBM were determined by absorption isotherm measurements. In this study, the CBMs of animal cellulases were expressed and characterized for the first time.
学科主题	Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Chemistry; Food Science & Technology
类目[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry, Applied ; Food Science & Technology
关键词[WOS]	CELLULOSE-BINDING DOMAIN ; PICHIA-PASTORIS ; XYLANASE ; FAMILY ; SPECIFICITY ; CLONING ; PURIFICATION ; MECHANISM ; RESIDUES ; MOLLUSK
收录类别	SCI
语种	英语
WOS记录号	WOS:000287991800008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/745]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yin, QY,Teng, YG,Li, YH,et al. Expression and Characterization of Full-Length Ampullaria crossean Endoglucanase EG65s and Their Two Functional Modules[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2011,75(2):240-246.
APA	Yin, QY,Teng, YG,Li, YH,Ding, M,&Zhao, FK.(2011).Expression and Characterization of Full-Length Ampullaria crossean Endoglucanase EG65s and Their Two Functional Modules.BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,75(2),240-246.
MLA	Yin, QY,et al."Expression and Characterization of Full-Length Ampullaria crossean Endoglucanase EG65s and Their Two Functional Modules".BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 75.2(2011):240-246.