AP1 is essential for generation of autophagosomes from the trans-Golgi network

	AP1 is essential for generation of autophagosomes from the trans-Golgi network
	Guo, YJ; Chang, CM; Huang, R; Liu, B; Bao, L; Liu, W
刊名	JOURNAL OF CELL SCIENCE
	2012
卷号	125 期号:7 页码:1706-1715
关键词	AP1 LC3 Autophagosome Membrane trafficking Trans-Golgi network
通讯作者	Liu, W (reprint author), Zhejiang Univ, Sch Med, Dept Biochem & Mol Biol, Program Mol & Cell Biol, Hangzhou 310058, Zhejiang, Peoples R China.,liuwei666@zju.edu.cn
英文摘要	Despite recent advances in understanding the functions of autophagy in developmental and pathological conditions, the underlying mechanism of where and how autophagosomal structures acquire membrane remains enigmatic. Here, we provide evidence that post-Golgi membrane traffic plays a crucial role in autophagosome formation. Increased secretion of constitutive cargo from the trans-Golgi network (TGN) to the plasma membrane induced the formation of microtubule-associated protein light chain 3 (LC3)-positive structures. At the early phase of autophagy, LC3 associated with and then budded off from a distinct TGN domain without constitutive TGN-to-plasma cargo and TGN-to-endosome proteins. The clathrin adaptor protein AP1 and clathrin localized to starvation-and rapamycin-induced autophagosomes. Dysfunction of the AP1-dependent clathrin coating at the TGN but not at the plasma membrane prevented autophagosome formation. Our results thus suggest an essential role of the TGN in autophagosome biogenesis, providing membrane to autophagosomes through an AP1-dependent pathway.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	ENDOPLASMIC-RETICULUM ; PLASMA-MEMBRANE ; SACCHAROMYCES-CEREVISIAE ; SEC PROTEINS ; LIVING CELLS ; SECRETORY PATHWAY ; VESICLE FORMATION ; CLATHRIN ; ER ; COMPARTMENTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000303911300011
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/627]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Guo, YJ,Chang, CM,Huang, R,et al. AP1 is essential for generation of autophagosomes from the trans-Golgi network[J]. JOURNAL OF CELL SCIENCE,2012,125(7):1706-1715.
APA	Guo, YJ,Chang, CM,Huang, R,Liu, B,Bao, L,&Liu, W.(2012).AP1 is essential for generation of autophagosomes from the trans-Golgi network.JOURNAL OF CELL SCIENCE,125(7),1706-1715.
MLA	Guo, YJ,et al."AP1 is essential for generation of autophagosomes from the trans-Golgi network".JOURNAL OF CELL SCIENCE 125.7(2012):1706-1715.