A simple and fast method for profiling microRNA expression from low-input total RNA by microarray

	A simple and fast method for profiling microRNA expression from low-input total RNA by microarray
	Zhao, BT; Jin, L; Wei, JL; Ma, ZL; Jiang, WJ; Ma, LJ; Jin, YX
刊名	IUBMB LIFE
	2012
卷号	64 期号:7 页码:612-616
关键词	eukaryotic gene expression
通讯作者	Zhao, BT (reprint author), Shanghai Univ, Sch Life Sci, 333 Nanchen Rd, Shanghai 200444, Peoples R China.,zhaobotao@shu.edu.cn ; ljma5@yahoo.com
英文摘要	Analysis of mature microRNA (miRNA) expression is important to understand its physiological functions and pathological implications. Microarray is a powerful technology to profile global miRNA expression. In this study, we developed a rapid miRNA labeling method by which miRNA was directly labeled in total RNA for microarray detection. This method consists of RNA tailing by poly(A) polymerase, reverse transcription, and template-switching by moloney murine leukemia virus (MMLV) reverse transcriptase. After these reactions, the small RNA cDNA was ready for labeling for microarray detection. The whole process of prearray sample preparation was dramatically shortened to 2 h. Furthermore, this method allows very limited starting total RNA (100 ng) for microarray analysis. Our data showed that the results from our method were highly consistent with that of real-time polymerase chain reaction (PCR). 2012 IUBMB IUBMB Life, 64(7): 612616, 2012
学科主题	Biochemistry & Molecular Biology; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	CANCER ; BIOMARKERS ; DISEASES ; SERUM
收录类别	SCI
语种	英语
WOS记录号	WOS:000305333200005
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/624]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhao, BT,Jin, L,Wei, JL,et al. A simple and fast method for profiling microRNA expression from low-input total RNA by microarray[J]. IUBMB LIFE,2012,64(7):612-616.
APA	Zhao, BT.,Jin, L.,Wei, JL.,Ma, ZL.,Jiang, WJ.,...&Jin, YX.(2012).A simple and fast method for profiling microRNA expression from low-input total RNA by microarray.IUBMB LIFE,64(7),612-616.
MLA	Zhao, BT,et al."A simple and fast method for profiling microRNA expression from low-input total RNA by microarray".IUBMB LIFE 64.7(2012):612-616.