NRSF/REST is required for gastrulation and neurogenesis during zebrafish development

	NRSF/REST is required for gastrulation and neurogenesis during zebrafish development
	Wang, XS; Ren, JK; Wang, ZG; Yao, JH; Fei, J
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2012
卷号	44 期号:5 页码:385-393
关键词	NRSF REST in situ hybridization morpholino maternal gastrulation neurogenesis
通讯作者	Yao, JH (reprint author), Fudan Univ, State Key Lab Genet Engn, Inst Genet, Sch Life Sci, Shanghai 200433, Peoples R China.,yaojh@fudan.edu.cn ; jfei@tongji.edu.cn
英文摘要	Repressor element 1-silencing transcription factor (REST) was recognized as a transcription suppressor regulating nervous system differentiation. However, the role of REST during early development has not been clarified. We cloned the zebrafish homolog of human REST. Real-time polymerase chain reaction results showed that zebrafish REST mRNA was both maternal and zygotic with the higher expression level from blastula to the late segmentation period. Whole-mount in situ hybridization showed that REST was strongly expressed in the blastoderm since dome stage and dynamically expressed mainly in developing brain, especially in the border of the brain subdivisions in early segmentation period. Knockdown of REST using translation blocking morpholino (MO-tra) technique resulted in gastrulation delay or even blockage, and subsequently led to embryo lethality by early segmentation period with deficient neurogenesis. However, splicing blocking morpholino for REST did not show obviously abnormal phenotype until 48 hpf (hours post-fertilization), indicating that maternal REST was an important regulator for gastrulation. Further study revealed that the abnormal development in MO-tra morphants was at least partly due to the dysfunction of protein transportation from the yolk to the blastoderm. Our results showed that REST (especially maternal supplied REST) was required for gastrulation and neurogenesis during zebrafish early embryogenesis.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	EMBRYONIC STEM-CELLS ; TARGET GENES ; FACTOR REST/NRSF ; REST ; EXPRESSION ; REPRESSOR ; SILENCER ; DIFFERENTIATION ; CHROMATIN ; NEURONS
收录类别	SCI
语种	英语
WOS记录号	WOS:000303333800002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/599]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, XS,Ren, JK,Wang, ZG,et al. NRSF/REST is required for gastrulation and neurogenesis during zebrafish development[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2012,44(5):385-393.
APA	Wang, XS,Ren, JK,Wang, ZG,Yao, JH,&Fei, J.(2012).NRSF/REST is required for gastrulation and neurogenesis during zebrafish development.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,44(5),385-393.
MLA	Wang, XS,et al."NRSF/REST is required for gastrulation and neurogenesis during zebrafish development".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 44.5(2012):385-393.