Thrombin Receptor Regulates Hematopoiesis and Endothelial-to-Hematopoietic Transition

	Thrombin Receptor Regulates Hematopoiesis and Endothelial-to-Hematopoietic Transition
	Yue, R; Li, HS; Liu, H; Li, YY; Wei, B; Gao, G; Jin, Y; Liu, TX; Wei, LP; Du, JL
刊名	DEVELOPMENTAL CELL
	2012
卷号	22 期号:5 页码:1092-1100
通讯作者	Pei, G (reprint author), Chinese Acad Sci, State Key Lab Cell Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci,Grad Sch, Shanghai 200031, Peoples R China.,gpei@sibs.ac.cn
英文摘要	Hematopoietic development and vascular development are closely related physiological processes during vertebrate embryogenesis. Recently, endothelial-to-hematopoietic transition (EHT) was demonstrated to be critical for hematopoietic stem and progenitor cell induction, but its underlying regulatory mechanisms remain poorly understood. Here we show that thrombin receptor (F2r), a protease-activated G protein-coupled receptor required for vascular development, functions as a negative regulator during hematopoietic development. F2r is significantly upregulated during hematopoietic differentiation of mouse embryonic stem cells (mESCs) and zebrafish hematopoietic development. Pharmacological or genetic inhibition of F2r promotes hematopoietic differentiation, whereas F2r overexpression shows opposite effects. Further mechanistic studies reveal that F2r-RhoA/ROCK pathway inhibits EHT in vitro and negatively regulates zebrafish EHT and hematopoietic stem cell induction in vivo. Taken together, this study demonstrates a fundamental role of F2r-RhoA/ROCK pathway in vertebrate hematopoiesis and EHT, as well as an important molecular mechanism coordinating hematopoietic and vascular development.
学科主题	Cell Biology; Developmental Biology
类目[WOS]	Cell Biology ; Developmental Biology
关键词[WOS]	PROTEASE-ACTIVATED RECEPTORS ; EPITHELIAL-CELL PLASTICITY ; STEM-CELLS ; DEFINITIVE HEMATOPOIESIS ; EMBRYONIC-DEVELOPMENT ; AORTIC ENDOTHELIUM ; COUPLED RECEPTORS ; HAEMOGENIC ENDOTHELIUM ; ZEBRAFISH EMBRYO ; RHO-GTPASES
收录类别	SCI
语种	英语
WOS记录号	WOS:000304291700020
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/594]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yue, R,Li, HS,Liu, H,et al. Thrombin Receptor Regulates Hematopoiesis and Endothelial-to-Hematopoietic Transition[J]. DEVELOPMENTAL CELL,2012,22(5):1092-1100.
APA	Yue, R.,Li, HS.,Liu, H.,Li, YY.,Wei, B.,...&Pei, G.(2012).Thrombin Receptor Regulates Hematopoiesis and Endothelial-to-Hematopoietic Transition.DEVELOPMENTAL CELL,22(5),1092-1100.
MLA	Yue, R,et al."Thrombin Receptor Regulates Hematopoiesis and Endothelial-to-Hematopoietic Transition".DEVELOPMENTAL CELL 22.5(2012):1092-1100.