Highly Efficient Derivation of Skeletal Myotubes from Human Embryonic Stem Cells

	Highly Efficient Derivation of Skeletal Myotubes from Human Embryonic Stem Cells
	Rao, LJ; Tang, WJ; Wei, YZ; Bao, L; Chen, JJ; Chen, HD; He, LXZ; Lu, PF; Ren, JT; Wu, L
刊名	STEM CELL REVIEWS AND REPORTS
	2012
卷号	8 期号:4 页码:1109-1119
关键词	Human embryonic stem cells Skeletal myotube Directed differentiation Muscle development Tet-on MyoD
通讯作者	Xiao, L (reprint author), Zhejiang Univ, Stem Cell & Dev Biol Res Ctr, Coll Anim Sci, 866 Yuhangtang Rd,Nongshenghuan Bldg E,Room 301, Hangzhou 310058, Zhejiang, Peoples R China.,leixiao@sibs.ac.cn
英文摘要	Human embryonic stem cells (hESCs) are a promising model for the research of embryonic development and regenerative medicine. Since the first hESC line was established, many researchers have shown that pluripotent hESCs can be directed into many types of functional adult cells in culture. However, most of the reported methods have induced differentiation through the alteration of growth factors in the culture medium. These methods are time consuming; moreover, it is difficult to obtain a pure population of the desired cells because of the low efficiency of induction. In this study, we used a lentiviral-based inducible gene-expression system in hESCs to control the ectopic expression of MyoD, which is an essential transcription factor in skeletal muscle development. The induction of MyoD can efficiently direct the pluripotent hESCs into mesoderm in 24 h. The cells then become proliferated myoblasts and finally form multinucleated myotubes in vitro. The whole procedure took about 10 days, with an induction efficiency of over 90 %. To our knowledge, this is the first time that hESCs have been induced into terminally differentiated cells with only one factor. In the future, these results could be a potential resource for cell therapy for diseases of muscle dysfunction.
学科主题	Cell Biology; Research & Experimental Medicine
类目[WOS]	Cell & Tissue Engineering ; Cell Biology ; Medicine, Research & Experimental
关键词[WOS]	SATELLITE CELLS ; IN-VITRO ; TERMINAL DIFFERENTIATION ; TRANSGENE EXPRESSION ; HUMAN ES ; MUSCLE ; GENE ; MYOD ; CULTURE ; MOUSE
收录类别	SCI
语种	英语
WOS记录号	WOS:000311510600009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/571]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Rao, LJ,Tang, WJ,Wei, YZ,et al. Highly Efficient Derivation of Skeletal Myotubes from Human Embryonic Stem Cells[J]. STEM CELL REVIEWS AND REPORTS,2012,8(4):1109-1119.
APA	Rao, LJ.,Tang, WJ.,Wei, YZ.,Bao, L.,Chen, JJ.,...&Xiao, L.(2012).Highly Efficient Derivation of Skeletal Myotubes from Human Embryonic Stem Cells.STEM CELL REVIEWS AND REPORTS,8(4),1109-1119.
MLA	Rao, LJ,et al."Highly Efficient Derivation of Skeletal Myotubes from Human Embryonic Stem Cells".STEM CELL REVIEWS AND REPORTS 8.4(2012):1109-1119.