Human cytoplasmic ProX edits mischarged tRNA(Pro) with amino acid but not tRNA specificity

	Human cytoplasmic ProX edits mischarged tRNA(Pro) with amino acid but not tRNA specificity
	Ruan, LL; Zhou, XL; Tan, M; Wang, ED
刊名	BIOCHEMICAL JOURNAL
	2013
卷号	450 期号:1 页码:243-252
关键词	aminoacyl-tRNA synthetase ProX specificity determinant tRNA(Pro) tRNA editing tRNA specificity
通讯作者	Wang, ED (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Ctr RNA Res, Inst Biochem & Cell Biol,Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,edwang@sibcb.ac.cn
英文摘要	aaRSs (aminoacyl-tRNA synthetases) are responsible for ensuring the fidelity of the genetic code translation by accurately linking a particular amino acid to its cognate tRNA isoacceptor. To ensure accuracy of protein biosynthesis, some aaRSs have evolved an editing process to remove mischarged tRNA. The hydrolysis of the mischarged tRNA usually occurs in an editing domain, which is inserted into or appended to the main body of the. aaRS. In addition, autonomous, editing domain-homologous proteins can also trans-edit mischarged tRNA in concert or in compensating for the editing function of its corresponding aaRS. The freestanding ProX is a homologue of the editing domain of bacterial ProRS (prolyl-tRNA synthetase). In the present study, we cloned for the first time a gene encoding HsProX (human cytoplasmic ProX) and purified the expressed recombinant protein. The catalytic specificity of HsProX for non-cognate amino acids and identity elements on tRNA(Pro) for editing were also investigated. We found that HsProX could deacylate mischarged Ala-tRNA(Pro), but not Cys-HstRNA(UGG),(Pro) G, and specifically targeted the alanine moiety of Ala-tRNA(Pro). The importance of the CCA(76) end of the tRNA for deacylation activity and key amino acid residues in HsProX for its editing function were also identified.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	SYNTHETASE INSERTION DOMAIN ; HAEMOPHILUS-INFLUENZAE YBAK ; QUALITY-CONTROL ; CP1 DOMAIN ; PROTEIN-SYNTHESIS ; CELL VIABILITY ; GENETIC-CODE ; IN-VITRO ; DISCRIMINATION ; AMINOACYLATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000314322600024
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/520]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Ruan, LL,Zhou, XL,Tan, M,et al. Human cytoplasmic ProX edits mischarged tRNA(Pro) with amino acid but not tRNA specificity[J]. BIOCHEMICAL JOURNAL,2013,450(1):243-252.
APA	Ruan, LL,Zhou, XL,Tan, M,&Wang, ED.(2013).Human cytoplasmic ProX edits mischarged tRNA(Pro) with amino acid but not tRNA specificity.BIOCHEMICAL JOURNAL,450(1),243-252.
MLA	Ruan, LL,et al."Human cytoplasmic ProX edits mischarged tRNA(Pro) with amino acid but not tRNA specificity".BIOCHEMICAL JOURNAL 450.1(2013):243-252.