Bioluminescence imaging for IL-1 beta expression in experimental colitis

	Bioluminescence imaging for IL-1 beta expression in experimental colitis
	Li, LM; Liu, ZZ; Yang, XY; Yan, HM; Bao, SS; Fei, J
刊名	JOURNAL OF INFLAMMATION-LONDON
	2013
卷号	10 期号:1 页码:16-16
通讯作者	Bao, SS (reprint author), Tongji Univ, Sch Life Sci & Technol, Shanghai 200092, Peoples R China.,bob.bao@sydney.edu.au ; jfei124@126.com
英文摘要	Background: Interleukin 1 beta (IL-1 beta) contributes to the development of inflammatory bowel disease (IBD) and is correlated with the severity of intestinal inflammation. However, the precise source of IL-1 beta producing cells in DSS colitis is currently not known. Methods: To determine IL-1 beta activity during intestinal inflammation in real time, an IL-1 beta transgenic mouse has been generated by incorporating the firefly luciferase gene driven by a 4.5-kb fragment of human IL-1 beta gene promoter (named cHS4I-hIL-1 beta P-Luc transgenic mice). Dextran sodium sulfate (DSS) induced colitis was confirmed with clinical presentation and histopathology. Results: A substantial increase in luciferase activity (reflecting IL-1 beta production) in the region of inflamed colon was observed in a time dependent manner, followed by additional activity in the region of the mesenteric lymph node. The up-regulated luciferase activity was suppressed by dexamethasone (steroids) during DSS challenge, consistent with reduced severity of colitis, confirming the specificity of luciferase activity. Conclusions: Our data suggests that bioluminescence is an interesting technology, which may be used to evaluate transcription of various genes in real time in experimental colitis.
学科主题	Immunology
类目[WOS]	Immunology
关键词[WOS]	INFLAMMATORY-BOWEL-DISEASE ; SIGNAL-TRANSDUCTION PATHWAYS ; PROINFLAMMATORY CYTOKINES ; INTESTINAL INFLAMMATION ; MOUSE MODELS ; INTERLEUKIN-1 ; MICE ; STRESS ; CELLS ; COLON
收录类别	SCI
语种	英语
WOS记录号	WOS:000318607600002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/486]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, LM,Liu, ZZ,Yang, XY,et al. Bioluminescence imaging for IL-1 beta expression in experimental colitis[J]. JOURNAL OF INFLAMMATION-LONDON,2013,10(1):16-16.
APA	Li, LM,Liu, ZZ,Yang, XY,Yan, HM,Bao, SS,&Fei, J.(2013).Bioluminescence imaging for IL-1 beta expression in experimental colitis.JOURNAL OF INFLAMMATION-LONDON,10(1),16-16.
MLA	Li, LM,et al."Bioluminescence imaging for IL-1 beta expression in experimental colitis".JOURNAL OF INFLAMMATION-LONDON 10.1(2013):16-16.