The effect of the number of transferred embryos, the interval between nuclear transfer and embryo transfer, and the transfer pattern on pig cloning efficiency

	The effect of the number of transferred embryos, the interval between nuclear transfer and embryo transfer, and the transfer pattern on pig cloning efficiency
	Rim, CH; Fu, ZX; Bao, L; Chen, HD; Zhang, D; Luo, Q; Ri, HC; Huang, HF; Luan, ZD; Zhang, Y
刊名	ANIMAL REPRODUCTION SCIENCE
	2013
卷号	143 期号:1 页码:91-96
关键词	Nuclear transfer Embryo transfer Culture time Single oviduct Double oviduct
通讯作者	Jong, UM (reprint author), Zhejiang Univ, Sch Med, Coll Anim Sci, Hangzhou 310058, Zhejiang, Peoples R China.,jonguimyong@aliyun.com
英文摘要	To improve the efficiency of producing cloned pigs, we investigated the influence of the number of transferred embryos, the culturing interval between nuclear transfer (NT) and embryo transfer, and the transfer pattern (single oviduct or double oviduct) on cloning efficiency. The results demonstrated that transfer of either 150-200 or more than 200 NT embryos compared to transfer of 100-150 embryos resulted in a significantly higher pregnancy rate (48 +/- 16, 50 +/- 16 vs. 29 +/- 5%, p < 0.05) and average litter size (4.1 +/- 2.3, 7 +/- 3.6 vs. 2.5 +/- 0.5). In vitro culture of reconstructed embryos for a longer time (40 h vs. 20 h) resulted in higher (p < 0.05) pregnancy rate (44 9 vs. 31 +/- 3%) and delivery rate (44 9 vs. 25 +/- 9%). Furthermore, double oviductal transfer dramatically increased pregnancy rate (83 6 vs. 27 + 8%, p < 0.05), delivery rate (75 2 vs. 27 + 8%, p < 0.05) and average litter size (6.5 +/- 2.8 vs. 2.6 +/- 1.2) compared to single oviductal transfer. Our study demonstrated that an improvement in pig cloning efficiency is achieved by adjusting the number and in vitro culture time of reconstructed embryos as well as the embryo transfer pattern. (C) 2013 Elsevier B.V. All rights reserved.
学科主题	Agriculture; Reproductive Biology
类目[WOS]	Agriculture, Dairy & Animal Science ; Reproductive Biology
关键词[WOS]	ADULT SOMATIC-CELLS ; CLONED PIGS ; UTERINE CAPACITY ; PORCINE EMBRYOS ; PREGNANCY ; FETAL ; SWINE ; MAINTENANCE ; INTEGRATION ; ACTIVATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000329381600013
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/485]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Rim, CH,Fu, ZX,Bao, L,et al. The effect of the number of transferred embryos, the interval between nuclear transfer and embryo transfer, and the transfer pattern on pig cloning efficiency[J]. ANIMAL REPRODUCTION SCIENCE,2013,143(1):91-96.
APA	Rim, CH.,Fu, ZX.,Bao, L.,Chen, HD.,Zhang, D.,...&Jong, UM.(2013).The effect of the number of transferred embryos, the interval between nuclear transfer and embryo transfer, and the transfer pattern on pig cloning efficiency.ANIMAL REPRODUCTION SCIENCE,143(1),91-96.
MLA	Rim, CH,et al."The effect of the number of transferred embryos, the interval between nuclear transfer and embryo transfer, and the transfer pattern on pig cloning efficiency".ANIMAL REPRODUCTION SCIENCE 143.1(2013):91-96.